In this study, we investigated surface and biological properties of Ag–Sr‐doped mesoporous bioactive glass nanoparticle (Ag–Sr MBGN) loaded chitosan/gelatin coatings deposited by electrophoretic deposition (EPD) on 316L stainless steel. The EPD parameters, that is, deposition time, applied voltage, and distance between the electrodes was optimized by the Taguchi design of experiment (DoE) approach. Scanning electron microscopy (SEM) images illustrated the spherical morphology of the synthesized Ag–Sr MBGNs with the mean particle size of 160 ± 20 nm. Energy‐dispersive X‐ray (EDX) spectroscopy results confirmed the presence of Ag and Sr in the synthesized MBGNs. Optimum EPD parameters determined by DoE approach were 5 g/L of Ag–Sr MBGNs, deposition time of 5 min, and applied voltage of 30 V. SEM images confirmed that the coatings were fairly homogenous. Fourier‐transform infrared spectroscopy and EDX results confirmed the presence of chitosan, gelatin, and Ag–Sr MBGNs in the coatings. Chitosan/gelatin/Ag–Sr MBGN composite coatings exhibited suitable wettability for the protein attachment and proliferation of osteoblast cells. The composite coatings exhibited suitable adhesion strength with the substrate. The coatings developed HA crystals upon immersion in simulated body fluid. The results of the turbidity test confirmed that the coatings are antibacterial to the Escherichia coli cells.

中文翻译：

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掺有壳聚糖/明胶涂层的Ag-Sr掺杂介孔生物活性玻璃纳米颗粒用于骨科植入物

在这项研究中，我们研究了通过电泳沉积（EPD）在316L不锈钢上沉积的掺Ag-Sr的介孔生物活性玻璃纳米颗粒（Ag-Sr MBGN）负载的壳聚糖/明胶涂层的表面和生物学特性。通过Taguchi实验设计（DoE）方法优化了EPD参数，即沉积时间，施加电压和电极之间的距离。扫描电子显微镜（SEM）图像说明了合成的Ag-Sr MBGNs的球形形态，平均粒径为160±20 nm。能量色散X射线（EDX）光谱结果证实了合成的MBGN中存在Ag和Sr。通过DoE方法确定的最佳EPD参数为5 g / L的Ag–Sr MBGN，沉积时间为5分钟和施加的电压为30V。SEM图像证实涂层相当均匀。傅里叶变换红外光谱和EDX结果证实了涂层中存在壳聚糖，明胶和Ag-Sr MBGN。壳聚糖/明胶/ Ag-Sr MBGN复合涂层对蛋白质的附着和成骨细胞的增殖具有合适的润湿性。复合涂层表现出与基材的合适的粘合强度。当浸入模拟体液中时，涂层会形成HA晶体。浊度测试的结果证实了该涂料具有抗细菌性。壳聚糖/明胶/ Ag-Sr MBGN复合涂层对蛋白质的附着和成骨细胞的增殖具有合适的润湿性。复合涂层表现出与基材的合适的粘合强度。当浸入模拟体液中时，涂层会形成HA晶体。浊度测试的结果证实了该涂料具有抗细菌性。壳聚糖/明胶/ Ag-Sr MBGN复合涂层对蛋白质的附着和成骨细胞的增殖具有合适的润湿性。复合涂层表现出与基材的合适的粘合强度。当浸入模拟体液中时，涂层会形成HA晶体。浊度测试的结果证实了该涂料具有抗细菌性。大肠杆菌细胞。