Propagation of orchid ( Cymbidium tracyanum L. Castle) treated by using an air cold atmospheric pressure plasma jet at room temperature was investigated. The peak-to-peak voltage discharge and flow rate of the air atmospheric pressure plasma jet were set at 10.5 kV and 10 L/min, respectively. The constituent particles of the air cold atmospheric pressure plasma jet were revealed by optical emission spectroscopy. At the end of the jet nozzle, the constituent particles were observed, such as hydroxyl radical ˙OH, N 2 first negative system (N 2 1 −), N 2 s positive system (N 2 2 +), and atomic oxygen. The average size, average number of buds, fresh and dry weights simultaneously increased by cold air plasma treatment compared with the control ones. The highest positive effects of treated protocorms on average size, average number of buds, fresh and dry weight were significantly improved by 238.2, 475.9, 85.6, and 152.5%, respectively. The transmission electron microscope showed the cytoplasmic cell wall in orchid protocorm disturbed by air cold plasma. Thus, the effects of constituent particles of air plasma might attack in the cell wall of protocorm leading to cell loosening, which improved elongation and released bud dormancy. These results indicated that the propagation of orchid protocorms depended on the effects of air cold plasma treatment with different plasma time durations.

中文翻译：

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冷气压空气等离子射流促进兰花原球茎的繁殖（Cymbidium tracyanum L. Castle）

研究了在室温下使用空气冷常压等离子体射流处理的兰花（Cymbidium tracyanum L. Castle）的繁殖。空气常压等离子体射流的峰峰值电压放电和流速分别设置为 10.5 kV 和 10 L/min。通过光学发射光谱揭示了空气冷常压等离子体射流的组成颗粒。在喷嘴末端，观察到组成粒子，如羟基·OH、N 2 第一负系统（N 2 1 -）、N 2 s 正系统（N 2 2 +）和原子氧。与对照相比，冷空气等离子体处理的平均大小、平均芽数、鲜重和干重同时增加。处理过的原球茎对平均大小、平均芽数、鲜重和干重分别显着提高了 238.2、475.9、85.6 和 152.5%。透射电镜显示兰花原球茎的细胞质细胞壁受到空气冷等离子体的干扰。因此，空气等离子体的组成粒子的作用可能会攻击原球茎的细胞壁，导致细胞松动，从而提高伸长率并解除芽休眠。这些结果表明，兰花原球茎的繁殖取决于不同等离子体持续时间的空气冷等离子体处理的效果。空气等离子体的组成粒子可能会攻击原球茎的细胞壁，导致细胞松动，从而提高伸长率并解除芽休眠。这些结果表明，兰花原球茎的繁殖取决于不同等离子体持续时间的空气冷等离子体处理的效果。空气等离子体的组成粒子可能会攻击原球茎的细胞壁，导致细胞松动，从而提高伸长率并解除芽休眠。这些结果表明，兰花原球茎的繁殖取决于不同等离子体持续时间的空气冷等离子体处理的效果。