The current study was designed to evaluate the anti-oxidant and anti-arthritic potential of a traditionally used herb, Monotheca buxifolia. The M. buxifolia methanolic extract (MBME) was prepared from the aerial parts of the plant followed by chemical characterization with GC–MS. The anti-oxidant potential of the MBME was demonstrated by DPPH scavenging activity. The effects of MBME on protein denaturation and membrane stabilization were determined by inhibition of egg albumin denaturation and RBC membrane stabilization assays, respectively. The in vivo anti-arthritic potential of the MBME at 50, 100, and 150 mg/kg/day was evaluated in Complete Freund’s Adjuvant-induced polyarthritis in Wistar rats treated for 21 days. Phytochemicals, such as linolenic acid methyl ester, n-hexadecanoic acid, vitamin E, α-amyrin, and β-amyrin were detected in the GC–MS analysis. The plant extract exhibited a 55.20 ± 0.69% scavenging of free radicals at 100 µg/ml concentration. It significantly (p < 0.05) stabilized human RBC membrane (65.06 ± 0.22%) and inhibited protein denaturation (70.53 ± 0.34%) at 100 mg/ml concentration. The diclofenac sodium (DS) and MBME at 150,100, and 50 mg/kg reduced the paw edema, restored the body weight, and altered blood parameters including CRP. The MBME significantly reduced the MDA and increased the SOD, CAT, and GSH levels in liver tissue homogenate in treated rats. The serum concentration of TNF-α and PGE2 was remarkably (p < 0.01–< 0.0001) restored by the DS and MBME dose dependently. The histopathological study showed that MBME 150 mg/kg commendably restored the ankle joint inflammation, bone erosion, and cartilage damage in polyarthritic rats. It was concluded that the anti-oxidant, anti-inflammatory and anti-arthritic effects of MBME might be attributed to phenols, flavonoids, triterpenoids, vitamin E, phytol, and other fatty acids. This study showed the anti-arthritic potential of Monotheca buxifolia and thus validates its traditional claim.

目前的研究旨在评估一种传统使用的草本植物Monotheca buxifolia的抗氧化和抗关节炎潜力。该M. buxifolia从植物地上部分制备甲醇提取物 (MBME)，然后用 GC-MS 进行化学表征。MBME 的抗氧化潜力由 DPPH 清除活性证明。MBME 对蛋白质变性和膜稳定性的影响分别通过抑制卵白蛋白变性和 RBC 膜稳定性测定来确定。在治疗 21 天的 Wistar 大鼠中，在完全弗氏佐剂诱导的多关节炎中评估了 50、100 和 150 毫克/千克/天的 MBME 的体内抗关节炎潜力。在 GC-MS 分析中检测到植物化学物质，如亚麻酸甲酯、正十六烷酸、维生素 E、α-香树脂精和β-香树脂精。植物提取物在 100 µg/ml 浓度下表现出 55.20 ± 0.69% 的自由基清除率。它显着（p  < 0.05) 在 100 mg/ml 浓度下稳定人红细胞膜 (65.06 ± 0.22%) 并抑制蛋白质变性 (70.53 ± 0.34%)。150,100 和 50 mg/kg 的双氯芬酸钠 (DS) 和 MBME 减少了爪水肿，恢复了体重，并改变了包括 CRP 在内的血液参数。MBME 显着降低 MDA 并增加治疗大鼠肝组织匀浆中的 SOD、CAT 和 GSH 水平。血清TNF-α和PGE2浓度显着（p < 0.01–< 0.0001) 由 DS 和 MBME 剂量依赖性恢复。组织病理学研究表明，MBME 150 mg/kg 可以很好地恢复多关节炎大鼠的踝关节炎症、骨侵蚀和软骨损伤。结果表明，MBME 的抗氧化、抗炎和抗关节炎作用可能归因于酚类、黄酮类、三萜类、维生素 E、植醇和其他脂肪酸。这项研究显示了Montheca buxifolia的抗关节炎潜力，从而验证了其传统主张。