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Genome-wide association studies revealed novel stripe rust resistance QTL in barley at seedling and adult-plant stages
Euphytica ( IF 1.9 ) Pub Date : 2021-01-01 , DOI: 10.1007/s10681-020-02728-1
Sanjaya Gyawali , Sujan Mamidi , Shiaoman Chao , Subhash C. Bhardwaj , Pradeep S. Shekhawat , Rajan Selvakumar , Om P. Gangwar , Ramesh Pal Singh Verma

Barley stripe rust, Puccinia striiformis f. sp. hordei , (PSH) occurs worldwide and is one of the major barley diseases of South Asia and North Africa. The aim of this study was to identify and map rust resistance loci at the seedling and the adult plant stages in 336 barley genotypes assembled at ICARDA as AM-2014. The seedling resistance to PSH was evaluated in the greenhouse individually for six virulent races originated from India. The adult stage resistance to PSH was evaluated in field located at Durgapura, Rajasthan, India. The AM-2014 panel was genotyped with infinium iSelect 9K barley SNP markers and genome-wide association studies were carried out using mixed linear model accounting for population structure (PCA) and kinship (K) as covariates. Eleven barley genotypes were identified resistant to all six PSH races at the seedling stage while these genotypes were also resistant at adult plant stage in the field. The most effective SNP markers (at least one for each QTL) associated with rust resistance was also identified using stepwise regression. A total of 17 and 25 resistance QTL to six races of PSH were identified in two- and six-rowed barley at the seedling stage, respectively. While, six and seven resistance QTL to PSH were detected at the adult stage in two- and six-rowed barley, respectively. The novel PSH resistant QTL detected at the seedling and adult stages are important for barley breeding programs globally.

中文翻译:

全基因组关联研究揭示了大麦幼苗和成株阶段的新型条锈病抗性 QTL

大麦条锈病,Puccinia striiformis f. sp. hordei, (PSH) 发生在世界各地,是南亚和北非的主要大麦病害之一。本研究的目的是在 ICARDA 作为 AM-2014 组装的 336 个大麦基因型中,鉴定和绘制幼苗和成株阶段的抗锈病基因座。对源自印度的六个有毒小种在温室中分别评估了幼苗对 PSH 的抗性。在位于印度拉贾斯坦邦 Durgapura 的田间评估了成年阶段对 PSH 的抗性。AM-2014 panel 使用 infinium iSelect 9K 大麦 SNP 标记进行基因分型,并使用混合线性模型将种群结构 (PCA) 和亲属关系 (K) 作为协变量进行全基因组关联研究。在苗期鉴定出 11 个大麦基因型对所有 6 个 PSH 小种具有抗性,而这些基因型在田间成株阶段也具有抗性。还使用逐步回归确定了与防锈性相关的最有效的 SNP 标记(每个 QTL 至少有一个)。在幼苗期的二行和六行大麦中,分别鉴定出17个和25个抗PSH小种的17个和25个抗性QTL。同时,在二棱和六棱大麦的成年期分别检测到6和7抗PSH QTL。在幼苗和成虫阶段检测到的新型 PSH 抗性 QTL 对全球大麦育种计划非常重要。还使用逐步回归确定了与防锈性相关的最有效的 SNP 标记(每个 QTL 至少有一个)。在幼苗期的二行和六行大麦中,分别鉴定出17个和25个抗PSH小种的17个和25个抗性QTL。同时,在二棱和六棱大麦的成年期分别检测到6和7抗PSH QTL。在幼苗和成虫阶段检测到的新型 PSH 抗性 QTL 对全球大麦育种计划非常重要。还使用逐步回归确定了与防锈性相关的最有效的 SNP 标记(每个 QTL 至少有一个)。在幼苗期的二行和六行大麦中,分别鉴定出17个和25个抗PSH小种的17个和25个抗性QTL。同时,在二棱和六棱大麦的成年期分别检测到6和7抗PSH QTL。在幼苗和成虫阶段检测到的新型 PSH 抗性 QTL 对全球大麦育种计划非常重要。
更新日期:2021-01-01
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