Roselle (Hibiscus sabdariffa L.) is one of the important medicinal herbs with diverse therapeutic properties. A number of bacteria and fungi can stimulate plant as growth‐promoting microorganisms. To assay the nutritional influence of three Pseudomonas bacteria (P. fluorescens, P. putida, P. aeruginosa) and two Trichoderma rifaii (wild type and mutant) strains on the morpho-physiological properties of roselle, a factorial experiment was performed in a completely randomized block design with four replications at two locations. The results showed that the plant height (up to 1.2-fold), fresh/dry weight of aerial parts (up to 1.6-fold), number, and fresh/dry weight of bolls (up to 1.3-fold) were significantly increased by the Pseudomonas spp. and T. rifaii treatments when compared to the control. The chlorophyll content (up to 2.2-fold) and anthocyanin content (up to 3.3-fold) along with catalase activity (up to 3.1-fold) and guaiacol peroxidase activity (up to 2.2-fold) were enhanced by the bacteria and fungi when compared to the control. P. fluorescens, P. putida, P. aeruginosa had the highest effect on the roselle, respectively. The combinatorial use of Pseudomonas spp. and T. rifaii had a greater influence on the improvement of the roselle properties studied than their use alone. Accordingly, the bio-fertilizer management using plant growth-promoting P. putida/P. aeruginosa bacteria and T. rifaii fungus can be an appropriate strategy to increase the yield of roselle. Further, the current study gives a novel insight into the synergistic effect of bacteria and fungi on the plant performance.

玫瑰茄（Roselle（Hibiscus sabdariffa L.））是具有多种治疗特性的重要草药之一。许多细菌和真菌可以刺激植物作为促进生长的微生物。为了测定3种的营养影响假单胞菌细菌（荧光假单胞菌，恶臭假单胞菌，铜绿假单胞菌）和两个木霉rifaii（野生型和突变型）菌株对玫瑰茄的形态生理特性的影响，在完全随机的区组设计中进行了析因实验，并在两个位置进行了四次重复。结果表明，株高（最高1.2倍），地上部分的鲜重/干重（最高1.6倍），棉铃数量和鲜重/干重（最高1.3倍）显着增加。在假单胞菌属。与对照组相比，T。rifaii处理。当细菌和真菌在以下条件下时，叶绿素含量（最高2.2倍）和花青素含量（最高3.3倍）以及过氧化氢酶活性（最高3.1倍）和愈创木酚过氧化物酶活性（最高2.2倍）得到增强。与对照相比。荧光假单胞菌，恶臭假单胞菌，铜绿假单胞菌对玫瑰茄的影响最高。假单胞菌属菌种的组合使用。比起单独使用，T。rifaii和T. rifaii对所研究的玫瑰茄性能的改善影响更大。因此，使用促进植物生长的恶臭假单胞菌/铜绿假单胞菌细菌和利福氏木霉菌的生物肥料管理可能是提高玫瑰茄产量的合适策略。此外，当前的研究为细菌和真菌对植物生长的协同作用提供了新的见解。