The population structure of Pseudomonas aeruginosa is panmictic-epidemic in nature, with the prevalence of some high-risk clones. These clones are often linked to virulence, antibiotic resistance, and more morbidity. The clonal success of these lineages has been linked to acquisition and spread of mobile genetic elements. The main aim of the study was to explore other molecular markers that explain their global success. A comprehensive set of 528 completely sequenced P. aeruginosa genomes was analyzed. The population structure was examined using Multilocus Sequence Typing (MLST). Strain relationships analysis and diversity analysis were performed using the geoBURST Full Minimum Spanning Tree (MST) algorithm and hierarchical clustering. A phylogenetic tree was constructed using the Unweighted Pair Group Method with Arithmetic mean (UPGMA) algorithm. A panel of previously investigated resistance markers were examined for their link to high-risk clones. A novel panel of molecular markers has been identified in relation to risky clones including armR, ampR, nalC, nalD, mexZ, mexS, gyrAT83I, gyrAD87N, nalCE153Q, nalCS46A, parCS87W, parCS87L, ampRG283E,ampRM288R, pmrALeu71Arg, pmrBGly423Cys, nuoGA890T, pstBE89Q, phoQY85F, arnAA170T, arnDG206C, and gidBE186A. In addition to mobile genetic elements, chromosomal variants in membrane proteins and efflux pump regulators can play an important role in the success of high-risk clones. Finding risk-associated markers during molecular surveillance necessitates applying more infection-control precautions.

中文翻译：

---

与铜绿假单胞菌流行高危克隆相关的新型分子标记

铜绿假单胞菌的种群结构在本质上是大流行性流行，并流行一些高危克隆。这些克隆通常与毒力，抗生素抗性和更高的发病率有关。这些谱系的克隆成功与移动遗传元件的获取和传播有关。该研究的主要目的是探索其他解释其全球成功的分子标记。一套完整的528个完全测序的铜绿假单胞菌基因组进行了分析。使用多基因座序列分型（MLST）检查种群结构。使用geoBURST全最小生成树（MST）算法和分层聚类进行了应变关系分析和多样性分析。使用带算术平均值的非加权对组方法（UPGMA）算法构建了系统树。检查了一组先前研究的抗性标记与高风险克隆的联系。已确定了一系列与风险克隆相关的新型分子标记，包括臂R，amp R，nal C，nal D，mex Z，mexS，gyr AT83I，gyr AD87N，nal CE153Q，nal CS46A，par CS87W，par CS87L，amp RG283E，amp RM288R，pmr ALeu71Arg，pmrBGly423Cys， nuo GA890T，pst BE89Q，pho QY85F，arn AA170T，arn DG206C和gid BE186A。除了可移动的遗传元件外，膜蛋白和外排泵调节剂中的染色体变异体在高风险克隆成功中也起着重要作用。在分子监测过程中发现与风险相关的标记物需要采取更多的感染控制预防措施。