Fusarium oxysporum, a fungal plant pathogen, causes severe wilting and heavy losses in cotton. Present research was planned to appraise the weed extracts of Parthenium hysterophorus, Chenopodium album, Canada thistle and Phalaris minor against F. oxysporum. Morphological identification of F. oxysporum was done by observing white cottony mycelium with dark-purple undersurface on growth media and oval to ellipsoid/kidney shaped oval tapering and three septate spores. Molecular characterization was done by amplifying internal transcribed spacer region using the ITS universal primers, ITS1 and ITS4. The weed extract with concentrations of 5%, 10%, 15% and 20% were applied by using food poison techniques under complete randomized design. Data was taken 3, 5 and 7 days after inoculation of F. oxysporum on potato dextrose agar (PDA). P. hysterophorus showed maximum antifungal response (97%) against F. oxysporum whereas other treatments effectively inhibited the pathogen growth on PDA media. Tebuconazole, a fungicide, was used as positive control. Trichoderma harzianum showed 98% inhibition of F. oxysporum on PDA. Consortium of Trichoderma harzianum + weed extracts was applied in infected roots of cotton grown in pots under complete randomized design. No disease was observed in treatment P. hysterophorus + T. harzianum whereas maximum disease was calculated (50%) in other treatments as compared to control (100%).

尖孢镰刀菌（Fusarium oxysporum）是一种真菌植物病原体，会导致严重的萎和棉花的严重损失。目前的研究是计划评价的杂草提取银胶菊，藜，加拿大蓟和虉次要对尖孢镰刀菌。尖孢镰刀菌的形态鉴定通过观察白色棉质菌丝体，在生长培养基上观察到暗紫色的下表面，并形成椭圆形至椭圆形/肾形的椭圆形锥形和三个分隔的孢子。通过使用ITS通用引物ITS1和ITS4扩增内部转录的间隔区来完成分子鉴定。在完全随机设计下，采用食物毒药技术施用浓度为5％，10％，15％和20％的杂草提取物。在马铃薯葡萄糖琼脂（PDA）上接种尖孢镰刀菌后3、5和7天获取数据。猪疫杆菌对尖孢镰刀菌表现出最大的抗真菌反应（97％），而其他治疗有效地抑制了PDA培养基上的病原体生长。戊唑醇（杀菌剂）用作阳性对照。哈茨木霉显示PDA上对尖孢镰刀菌有98％的抑制作用。 在完全随机设计下，将哈茨木霉+杂草提取物的联合体应用于盆栽棉花的感染根中。在P. hysterophorus + T. harzianum处理中未观察到疾病，而与对照（100％）相比，在其他处理中计算出最大疾病（50％）。