The objective of this study was to evaluate effects of dehydration on sperm DNA with the aim of eventually using this method for preserving llama spermatozoa. Two experiments were conducted: 1) sperm preservation at 5 °C for 60 days in different hyperosmotic solutions (500, 800, 1000 and 1200 mOsmol/l) (n = 6, replications = 2) and 2) sperm preservation at 5 and −20 °C for 60 days in the same hyperosmotic solutions, with supplementary antibiotics (n = 6, replications = 2). Sperm motility, membrane functional integrity, viability and morphology were evaluated at 0 and 48 h of the preservation period (Experiment 1) and at 30 min and 24 h (Experiment 2). Sperm DNA was evaluated at 0 or 30 min (Experiment 1 and 2, respectively) and on days 7, 14, 21, 30 and 60 of the preservation periods. Motility, membrane functional integrity and viability were less when sperm were dehydrated, while sperm cell morphology was not affected. There was a smaller percentage of sperm with condensed chromatin as duration of the preservation period increased when stored in the different hyperosmotic solutions. There was a markedly smaller (P < 0.05) percentage of sperm with intact DNA in all solutions as the duration of preservation increased, with there being greater values for intact DNA at −20 °C than sperm preserved at 5 °C. Llama sperm chromatin condensation was slightly affected by the process of dehydration. There was a markedly smaller percentage of sperm with intact DNA in the dehydrated semen samples.

本研究的目的是评估脱水对精子 DNA 的影响，目的是最终使用这种方法来保存美洲驼精子。进行了两个实验：1) 在不同的高渗溶液 (500、800、1000 和 1200 mOsmol/l) ( n = 6，重复 = 2) 和 2) 5°C 下保存精子 60 天和-在相同的高渗溶液中 20 °C 60 天，并添加抗生素 ( n= 6，重复 = 2）。在保存期的 0 和 48 小时（实验 1）以及 30 分钟和 24 小时（实验 2）评估精子活力、膜功能完整性、活力和形态。在 0 或 30 分钟（分别为实验 1 和 2）和保存期的第 7、14、21、30 和 60 天评估精子 DNA。当精子脱水时，运动性、膜功能完整性和活力降低，而精子细胞形态不受影响。当储存在不同的高渗溶液中时，随着保存期的持续时间增加，具有浓缩染色质的精子的百分比较小。明显变小（P< 0.05) 随着保存时间的增加，所有溶液中具有完整 DNA 的精子的百分比，在 -20 °C 下完整 DNA 的值比在 5 °C 下保存的精子值更高。羊驼精子染色质凝聚受脱水过程的影响较小。在脱水的精液样本中，具有完整 DNA 的精子百分比明显较低。