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FMT, a protein that affects mitochondrial distribution, interacts with translation-related proteins in Arabidopsis thaliana
Plant Cell Reports ( IF 6.2 ) Pub Date : 2021-01-01 , DOI: 10.1007/s00299-020-02634-9
Hiroki Ayabe 1 , Narumi Kawai 1 , Mitsuhiro Shibamura 1 , Yoichiro Fukao 2 , Masaru Fujimoto 1 , Nobuhiro Tsutsumi 1 , Shin-Ichi Arimura 1
Affiliation  

Key message

Two translation-related proteins are identified as FMT-interacting proteins. However, FMT, unlike mutants of other CLU genes in fly and human, has no clear impact on the accumulation of mitochondrial proteins.

Abstract

Organelle distribution is critical for effective metabolism and stress response and is controlled by various environmental factors. Clustered mitochondria (CLU) superfamily genes affect mitochondrial distribution and their disruptions cause mitochondria to cluster within a cell in various species including yeast, fly, mammals and Arabidopsis. In Arabidopsis thaliana, Friendly mitochondria (FMT) is a CLU gene that is required for normal mitochondrial distribution, but its molecular function is unclear. Here, we demonstrate that FMT interacts with some translation-related proteins (translation initiation factor eIFiso4G1 and glutamyl-tRNA synthetase OVA9), as well as itself. We also show FMT forms dynamic particles in the cytosol that sometimes move with mitochondria, and their movements are mainly controlled by actin filaments but also by microtubules. Similar results have been reported for animal CLU orthologs. However, an fmt mutant, unlike animal clu mutants, did not show any clear decrease of nuclear-encoded mitochondrial protein levels. This difference may reflect a functional divergence of FMT from other CLU superfamily genes.



中文翻译:

FMT,一种影响线粒体分布的蛋白质,与拟南芥中的翻译相关蛋白相互作用

关键信息

两种翻译相关蛋白被鉴定为 FMT 相互作用蛋白。然而,与苍蝇和人类中其他CLU基因的突变体不同,FMT 对线粒体蛋白的积累没有明显的影响。

抽象的

细胞器分布对于有效的新陈代谢和应激反应至关重要,并受各种环境因素的控制。成簇线粒体( CLU ) 超家族基因影响线粒体分布,它们的破坏导致线粒体在包括酵母、苍蝇、哺乳动物和拟南芥在内的各种物种的细胞内聚集。在拟南芥 中,友好 线粒体( FMT ) 是CLU线粒体正常分布所需的基因,但其分子功能尚不清楚。在这里,我们证明 FMT 与一些翻译相关蛋白(翻译起始因子 eIFiso4G1 和谷氨酰-tRNA 合成酶 OVA9)以及其自身相互作用。我们还显示 FMT 在胞质溶胶中形成动态粒子,这些粒子有时会随线粒体移动,它们的运动主要受肌动蛋白丝控制,但也受微管控制。动物CLU直系同源物也报道了类似的结果。然而,与动物clu突变体不同, fmt突变体没有显示出核编码线粒体蛋白水平的任何明显降低。这种差异可能反映了FMT与其他CLU的功能差异超家族基因。

更新日期:2021-01-01
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