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A novel quinoline-based turn-on fluorescent probe for the highly selective detection of Al (III) and its bioimaging in living cells, plants tissues and zebrafish
JBIC Journal of Biological Inorganic Chemistry ( IF 3 ) Pub Date : 2021-01-01 , DOI: 10.1007/s00775-020-01836-6
Wen Lu 1 , Jichao Chen 1 , Jiuzhou Shi 1 , Li Xu 1 , Shilong Yang 2 , Buhong Gao 2
Affiliation  

Abstract

A novel quinoline fluorescent probe QNP ((E)-N′-(5-chloro-2-hydroxybenzylidene) quinoline-2-carbohydrazide) for detection of Al3+ ion was designed, synthesized and characterized. QNP displayed a high fluorescence enhancement in the presence of Al3+ ion in DMF:PBS (99:1, v/v) solution and the detection limit was as low as 1.25 μM with high selectivity and excellent sensitivity from 0 to 3 μM. The sensing ability of QNP towards Al3+ ion is attributed to the synergistic effect of PET and ICT. Furthermore, the binding stoichiometry between QNP and Al3+ ion is of 1:1 by Job’s plot and mass spectrum, and the calculated binding constant is 4.29 × 108 M−1. The detection of Al3+ ion in water samples illustrates that QNP could be applied to the detection of practical samples in the environment. Bioimaging experiments on Hela cells, zebrafish and soybean root tissues demonstrate that it has potential application to investigate biological processes involving Al3+ ion within living cells.

Graphic abstract

A quinoline-based turn-on fluorescence probe for the detection of Al3+ and its bioimaging in living cells, plant, and zebrafish.



中文翻译:

一种新型喹啉基荧光探针,用于高选择性检测铝(III)及其在活细胞、植物组织和斑马鱼中的生物成像

摘要

设计、合成并表征了一种用于检测Al 3+离子的新型喹啉荧光探针QNP ((E)- N '-(5-chloro-2-hydroxybenzylidene) quinoline-2-carbohydrazide) 。QNP在 DMF:PBS (99:1, v / v ) 溶液中存在 Al 3+离子时显示出高荧光增强,检测限低至 1.25 μM,具有高选择性和 0 至 3 μM 的出色灵敏度。QNP对Al 3+离子的传感能力归因于PET和ICT的协同效应。此外,QNP和 Al 3+之间的结合化学计量根据乔布斯图和质谱图,离子为 1:1,计算的结合常数为 4.29 × 10 8  M -1。水样中Al 3+离子的检测表明QNP可以应用于环境中实际样品的检测。Hela 细胞、斑马鱼和大豆根组织的生物成像实验表明,它具有研究活细胞内涉及 Al 3+离子的生物过程的潜在应用。

图形摘要

一种基于喹啉的开启荧光探针,用于检测活细胞、植物和斑马鱼中的 Al 3+及其生物成像。

更新日期:2021-01-01
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