Protein phosphorylation catalyzed by protein kinases is the major regulatory mechanism that controls many cellular processes. The regulatory mechanism of one protein kinase in different signals is distinguished, probably inducing multiple phenotypes. The Saccharomyces cerevisiae Snf1 protein kinase, a member of the AMP‑activated protein kinase family, plays important roles in the response to nutrition and environmental stresses. Glucose is an important nutrient for life activities of cells, but glucose repression and osmotic pressure could be produced at certain concentrations. To deeply understand the role of Snf1 in the regulation of nutrient metabolism and stress response of S. cerevisiae cells, the role and the regulatory mechanism of Snf1 in glucose metabolism are discussed in different level of glucose: below 1% (glucose derepression status), in 2% (glucose repression status), and in 30% glucose (1.66 M, an osmotic equivalent to 0.83 M NaCl). In summary, Snf1 regulates glucose metabolism in a glucose-dependent manner, which is associated with the different regulation on activation, localization, and signal pathways of Snf1 by varied glucose. Exploring the regulatory mechanism of Snf1 in glucose metabolism in different concentrations of glucose can provide insights into the study of the global regulatory mechanism of Snf1 in yeast and can help to better understand the complexity of physiological response of cells to stresses.

由蛋白激酶催化的蛋白磷酸化是控制许多细胞过程的主要调节机制。一种蛋白激酶在不同信号中的调节机制是有区别的，可能会诱发多种表型。在酿酒酵母的Snf1蛋白激酶，腺苷酸活化蛋白激酶家族中的一员，扮演在应对营养和环境压力的重要作用。葡萄糖是细胞生命活动的重要营养物质，但在一定浓度下会产生葡萄糖抑制和渗透压。深入了解Snf1在调节酿酒酵母营养代谢和应激反应中的作用Snf1 在葡萄糖代谢中的作用和调节机制在不同葡萄糖水平下进行讨论：低于 1%（葡萄糖去抑制状态）、2%（葡萄糖抑制状态）和 30% 葡萄糖（1.66 M，渗透压相当于 0.83 M NaCl）。综上所述，Snf1 以葡萄糖依赖的方式调节葡萄糖代谢，这与不同葡萄糖对 Snf1 的激活、定位和信号通路的不同调节有关。探索不同浓度葡萄糖下Snf1在葡萄糖代谢中的调控机制，有助于深入研究Snf1在酵母中的全局调控机制，有助于更好地理解细胞对应激的生理反应的复杂性。