We investigated changes in 8-hydroxy-2-deoxyguanosine (8-OHdG) activity which is a product of oxidative DNA damage, histopathological changes and antioxidant responses in liver and gill tissues of rainbow trout, following a 21-day exposure to three different concentrations of linuron (30 µg/L, 120 µg/L and 240 µg/L). Our results indicated that linuron concentrations caused an increase in LPO levels of liver and gill tissues (p < 0.05). While linuron induced both increases and decreases in GSH levels and SOD activity, CAT activity was decreased by all concentrations of linuron (p < 0.05). The immunopositivity of 8-OHdG was detected in the hepatocytes of liver and in the epithelial and chloride cells of the secondary lamellae of the gill tissues. Our results suggested that linuron could cause oxidative DNA damage by causing an increase in 8-OHdG activity in tissues, and it induces histopathological damage and alterations in the antioxidant parameters of the tissues.

我们研究了 8-羟基-2-脱氧鸟苷 (8-OHdG) 活性的变化，该活性是虹鳟鱼肝脏和鳃组织中 DNA 氧化损伤、组织病理学变化和抗氧化反应的产物，在暴露于三种不同浓度后 21 天linuron（30 µg/L、120 µg/L 和 240 µg/L）。我们的结果表明，linuron 浓度导致肝脏和鳃组织的 LPO 水平增加（p < 0.05）。虽然 linuron 诱导 GSH 水平和 SOD 活性的增加和降低，但所有浓度的 linuron 都降低了 CAT 活性（p< 0.05)。在肝脏的肝细胞和鳃组织的次级薄片的上皮细胞和氯化物细胞中检测到8-OHdG的免疫阳性。我们的结果表明，linuron 可以通过引起组织中 8-OHdG 活性的增加来引起氧化性 DNA 损伤，并诱导组织病理学损伤和组织抗氧化参数的改变。