当前位置: X-MOL 学术J. Nat. Prod. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Discovery of Thanafactin A, a Linear, Proline-Containing Octalipopeptide from Pseudomonas sp. SH-C52, Motivated by Genome Mining
Journal of Natural Products ( IF 5.1 ) Pub Date : 2020-12-31 , DOI: 10.1021/acs.jnatprod.0c01174
Norbert Kirchner 1 , Carolina Cano-Prieto 1 , Anna-Christina Schulz-Fincke 2 , Michael Gütschow 2 , Nico Ortlieb 3, 4 , Julia Moschny 3 , Timo H J Niedermeyer 3, 4 , Jeannie Horak 5, 6 , Michael Lämmerhofer 5 , Menno van der Voort 7 , Jos M Raaijmakers 8, 9 , Harald Gross 1, 4
Affiliation  

Genome mining of the bacterial strains Pseudomonas sp. SH-C52 and Pseudomonas fluorescens DSM 11579 showed that both strains contained a highly similar gene cluster encoding an octamodular nonribosomal peptide synthetase (NRPS) system which was not associated with a known secondary metabolite. Insertional mutagenesis of an NRPS component followed by comparative profiling led to the discovery of the corresponding novel linear octalipopeptide thanafactin A, which was subsequently isolated and its structure determined by two-dimensional NMR and further spectroscopic and chromatographic methods. In bioassays, thanafactin A exhibited weak protease inhibitory activity and was found to modulate swarming motility in a strain-specific manner.

中文翻译:

发现 Thanafactin A,一种来自假单胞菌属的线性、含脯氨酸的八肽。SH-C52,受基因组挖掘启发

细菌菌株Pseudomonas sp. 的基因组挖掘。SH-C52 和荧光假单胞菌DSM 11579 表明,两种菌株都含有高度相似的基因簇,编码八模非核糖体肽合成酶 (NRPS) 系统,该系统与已知的次级代谢产物无关。NRPS 组分的插入诱变随后进行比较分析导致发现了相应的新型线性八脂肽 thanafactin A,随后将其分离并通过二维 NMR 和进一步的光谱和色谱方法确定其结构。在生物测定中,thanafactin A 表现出微弱的蛋白酶抑制活性,并被发现以菌株特异性方式调节蜂群运动。
更新日期:2021-01-22
down
wechat
bug