Disordered flexible linkers (DFLs) are abundant and functionally important intrinsically disordered regions that connect protein domains and structural elements within domains and which facilitate disorder-based allosteric regulation. Although computational estimates suggest that thousands of proteins have DFLs, they were annotated experimentally in <200 proteins. This substantial annotation gap can be reduced with the help of accurate computational predictors. The sole predictor of DFLs, DFLpred, trade-off accuracy for shorter runtime by excluding relevant but computationally costly predictive inputs. Moreover, it relies on the local/window-based information while lacking to consider useful protein-level characteristics.

中文翻译：

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APOD：无序柔性接头的基于序列的准确预测因子

无序的柔性接头（DFL）是大量且功能上很重要的内在无序区域，这些区域将蛋白质结构域和结构域内的结构元件连接起来，并促进基于疾病的变构调节。尽管计算估计表明成千上万的蛋白质具有DFL，但在实验中用<200种蛋白质对它们进行了注释。可以借助精确的计算预测变量来减少这种实质性的注释差距。DFL的唯一预测器DFLpred可以通过排除相关但计算成本高的预测输入来权衡精度，从而缩短运行时间。此外，它依赖于基于局部/窗口的信息，而又没有考虑有用的蛋白质水平特征。