ABSTRACT

Zingiberaceae species are widely in traditional medicine throughout the world. This study evaluates the potential biological applications of phyto-functionalised silver nanoparticles (AgNPs) synthesised using sprouted Zingiberaceae species. The AgNPs were characterised and found to be spherical in the size of 25 nm-30 nm. The experimental results indicated that AgNPs have notable bactericidal activity against gram-positive and gram-negative bacteria. Human colon cancer cells (HCT15) were used to perform the anticancer assay and cell viability at a concentration of 500 µg/mL ranged from 43.71% to 49.49%. Also sprouted C. amada AgNPs at a concentration of 200 μg/mL provided maximum inhibition of 65.40% and 68.78% for α-amylase and α-glucosidase activity respectively. Furthermore, the sprouted Z. officinale AgNPs at a concentration of 500 µg/mL displayed significant glucose uptake percentage (99.56%) under in vitro conditions. The results indicated that phyto-functionalised AgNPs from sprouted Z. officinale and C. amada possess antibacterial, anticancer and antidiabetic activity.

摘要

姜科物种广泛用于世界各地的传统医学中。本研究评估了使用发芽的姜科物种合成的植物功能化银纳米粒子 (AgNPs) 的潜在生物学应用。对 AgNPs 进行了表征，发现其呈球形，大小为 25 nm-30 nm。实验结果表明，AgNPs对革兰氏阳性菌和革兰氏阴性菌具有显着的杀菌活性。人结肠癌细胞 (HCT15) 用于进行抗癌试验，细胞活力在 500 µg/mL 的浓度范围为 43.71% 至 49.49%。还发芽了 C. amada浓度为 200 μg/mL 的 AgNPs 对 α-淀粉酶和 α-葡糖苷酶活性的最大抑制分别为 65.40% 和 68.78%。此外，在体外条件下，浓度为 500 µg/mL 的发芽的Z. officinale AgNPs 显示出显着的葡萄糖摄取百分比 (99.56%) 。结果表明，来自发芽的Z. officinale和C. amada的植物功能化 AgNPs具有抗菌、抗癌和抗糖尿病的活性。