In our article published in Plant Physiology, we had reported tarani (tni) mutant in Arabidopsis, in which poly-ubiquitin hydrolysis is adversely affected, shows pleiotropic phenotypic defects including fewer lateral roots due to the stabilization of several AUX/IAAs and reduced auxin response. TNI encodes UBIQUITIN-SPECIFIC PROTEASE14 that maintains normal auxin response through ubiquitin recycling. Fewer lateral roots observed in tni could be due to defects in their primordia initiation or subsequent elongation post-initiation. Here we have tested this by marking the lateral root primordia with pCycB1;1::CycB1;1(DB):GUS reporter and counting the number of lateral root at various stages development of as a marker of lateral root primordium. The results suggest that TNI/UBP14 is required for LRP development, and a reduction in TNI activity causes a delay in LRP initiation and consequently shorter lateral roots in the tni seedlings. ABBREVIATIONS: LRP, lateral root primordium; XPP, xylem pole pericycle; LRFC, lateral root founder cells.

中文翻译：

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拟南芥侧根发育需要TARANI/泛素蛋白酶14蛋白

在我们发表在植物生理学上的文章中，我们报道了拟南芥中的 tarani (tni) 突变体，其中多泛素水解受到不利影响，显示多效性表型缺陷，包括由于几种 AUX/IAAs 的稳定性和生长素反应降低而导致的侧根减少. TNI 编码 UBIQUITIN-SPECIFIC PROTEASE14，通过泛素回收维持正常的生长素反应。在 tni 中观察到的侧根较少可能是由于它们的原基起始缺陷或起始后的后续伸长。在这里，我们通过用 pCycB1;1::CycB1;1(DB):GUS 报告基因标记侧根原基并计算不同发育阶段的侧根数作为侧根原基的标记来测试这一点。结果表明 LRP 开发需要 TNI/UBP14，TNI 活性的降低会导致 LRP 启动延迟，从而导致 tni 幼苗的侧根变短。缩写：LRP，侧根原基；XPP，木质杆周轮；LRFC，侧根创始人细胞。