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Characterization, expression and function analysis of anthocyanidin reductase gene from Vitis vinifera L. cv. Cabernet Sauvignon under UV-C irradiation
Biotechnology & Biotechnological Equipment ( IF 1.4 ) Pub Date : 2020-12-30 , DOI: 10.1080/13102818.2020.1848460
Tiequan Niu 1, 2 , Changhen Li 1, 2 , Bo Yang 1, 2 , Pengfei Zhang 1, 2 , Weixin Fan 3 , Pengfei Wen 1, 2
Affiliation  

Abstract

Flavonoids are important polyphenols, and widely distributed in plants. Anthocyanidin reductase (ANR), a precursor enzyme in (-)- epicatechin synthesis, could play an important role in anthocyanins biosynthesis. In the present study, the ANR gene was cloned from Vitis vinifera L. cv. Cabernet Sauvignon. Compared with VvANR (GenBank Accession No. NM_001280956.1), they differed in only 4 bp and shared similarity of 99.6%. The ANR protein belongs to the cl33424 Superfamily. In addition, many light-responsive elements and abiotic stress-related elements (ABRE, MYB, WRKY, etc.) were found in the promoter region of the ANR gene. STRING analysis predicted that VvMYBPA1 and VvMYBPAR may be involved in regulating the expression of the ANR gene. Different UV-C irradiation times were used for Cabernet Sauvignon berries during grape berry development, and the comparison analysis showed that UV-C irradiation improved significantly the accumulation of catechin and epicatechin during 20–50 days after full bloom, especially, under UV-C irradiation for 5 min. Furthermore, UV-C irradiation for 5 min had a greater effect on the expression of the ANR gene, the accumulation of catechin and epicatechin, and the enzymatic activity of ANR than UV-C irradiation for 10 min. Through the virus-induced gene silencing system, the ANR-silenced grape leaves were chlorotic, in which the contents of catechin and epicatechin were both significantly lower than those in the control, showing a very significant difference, respectively. In summary, our results demonstrated that the ANR gene played a crucial role in the accumulation of epicatechin in grapes, and its expression was upregulated by UV-C irradiation.



中文翻译:

葡萄(Vitis vinifera L. cv。)花青素还原酶基因的表征,表达和功能分析 赤霞珠在紫外线辐射下

摘要

黄酮类化合物是重要的多酚,广泛分布于植物中。花青素还原酶(ANR)是(-)-表儿茶素合成的前体酶,可能在花青素的生物合成中起重要作用。在本研究中,ANR基因是从Vitis vinifera L. cv克隆的赤霞珠。与VvANR​​(GenBank登录号NM_001280956.1)相比,它们仅相差4 bp,共有相似性为99.6%。ANR蛋白属于cl33424超家族。另外,在ANR基因的启动子区域发现了许多光响应元件和非生物胁迫相关元件(ABRE,MYB,WRKY等)。STRING分析预测VvMYBPA1VvMYBPAR可能参与调节ANR基因的表达。赤霞珠浆果在葡萄果实发育过程中使用了不同的UV-C辐照时间,比较分析表明,UV-C辐照在盛开后20-50天(尤其是在UV-C下)显着改善了儿茶素和表儿茶素的积累。照射5分钟。此外,UV-C照射5分钟对ANR的表达有更大的影响该基因,儿茶素和表儿茶素的积累以及ANR的酶活性比UV-C辐照10分钟。通过病毒诱导的基因沉默系统,ANR沉默的葡萄叶片是褪绿的,儿茶素和表儿茶素的含量均显着低于对照,差异非常显着。总之,我们的结果表明,ANR基因在葡萄表儿茶素的积累中起着关键作用,并且其表达被UV-C辐射上调。

更新日期:2020-12-31
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