ABSTRACT

Agrimonia eupatoria L. (Rosaceae) as a valuable plant has been included in the International Union for Conservation of Nature (IUCN) Red List of threatened medicinal plants in some parts of the world. Because information on the development of reproductive organs is important to identify the species characteristics, in this study, the structure of stamen and gynoecium, developmental stages of the anther, ovule, male and female gametophyte, and embryo development in Agrimonia eupatoria were investigated using light and scanning electron microscopy. The results showed that the primary anther wall includes an epidermis, an endothecium, two or three rows of middle layer, and a glandular tapetum. The microspore tetrads are tetrahedral. Some of the tapetal cells are binucleated and mature pollen grains are two-celled. The pollen dispersal unit is the monad. Pollen grains are tricolporate, with striate exine. The ovules are hemi-anatropous, pendulous, unitegmic, and crassinucellate. Hypostase, funicular obturator, and nucellar cap were observed in the ovules. The functional megaspore in the linear megaspore tetrad is located in the micropylar zone. The development of the embryo sac was of Polygonum-type. The embryogenesis includes globular, transition, heart-shaped, and torpedo phases. Embryogeny of this plant is of the Asterad type. Haustorium endosperm was observed in the embryo sac.

摘要

龙牙耶夫帕托里亚L.（蔷薇科）作为一种有价值的植物已被列入国际联盟濒危药用植物的自然（IUCN）红色名录的保护在世界上的一些地方。由于有关生殖器官发育的信息对于确定物种特征很重要，因此在这项研究中，雄蕊和雌蕊的结构，花药，胚珠，雌雄配子体的发育阶段以及龙牙草的胚胎发育用光和扫描电子显微镜研究。结果表明，主花药壁包括一个表皮，一个内皮层，两排或三排中间层以及一个腺绒毛。小孢子四分体是四面体。一些绒毛细胞是双核的，成熟的花粉粒是两细胞的。花粉散布单位是单子。花粉粒是三孔孢子，外生有条纹。胚珠是半各向异性的，下垂的，同卵的和cr状的。在胚珠中观察到过氧化氢酶，缆索闭塞和细胞核帽。线性大孢子四元体中的功能性大孢子位于毛孔区域。胚囊的发育是何首乌-类型。胚胎发生包括球状，过渡，心形和鱼雷期。该植物的胚发生是Asterad类型的。在胚囊中观察到Haustorium胚乳。