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Comparison of the performance of aerosol sampling devices with aerosols containing Ebola virus
Aerosol Science and Technology ( IF 5.2 ) Pub Date : 2021-02-01 , DOI: 10.1080/02786826.2020.1867310
Michael A. Schuit 1 , Jill Taylor 1 , Rebecca Dunning 1 , David Miller 1 , Denise Freeburger 1 , Luis Faisca 1 , Victoria Wahl 1 , Paul A. Dabisch 1
Affiliation  

Abstract

Previous laboratory studies of Ebola virus aerosols have used a variety of different sampling devices to collect viral material for analysis. However, sampling devices vary in their ability to collect aerosol particles and preserve the infectivity of collected viruses. If not accounted for, this variability has the potential to bias study outcomes and confound comparisons between studies in which different samplers are used. In the present study, five laboratory-scale aerosol samplers were assessed for their ability to collect and preserve the infectivity of Ebola virus at low and high relative humidities. Relative humidity did not affect physical collection efficiencies, but infectivity was preserved better at high relative humidity for most samplers. Overall, 25-mm gelatin filters were identified as the optimal sampler among those tested based on their physical and biological efficiency, ease of use, lack of glass components, and ability to be recovered in small volumes to produce a more concentrated final sample. Tests demonstrated that 25-mm gelatin filters recovered in 2.5-mL media and assayed by microtitration using an Ebola reporter cell line resulted in an approximately 10-fold improvement in sensitivity compared to other published methods. The data provided by this study will be useful to facilitate comparisons between different studies utilizing aerosolized Ebola virus and to inform sampler selection in future studies.



中文翻译:

气溶胶采样装置与包含埃博拉病毒的气溶胶的性能比较

抽象的

以前对埃博拉病毒气溶胶的实验室研究已经使用了各种不同的采样设备来收集病毒材料进行分析。但是,采样设备收集气溶胶颗粒并保持所收集病毒的传染性的能力各不相同。如果不加以考虑,这种可变性可能会偏向研究结果,并会混淆使用不同采样器的研究之间的比较。在本研究中,评估了五个实验室规模的气溶胶采样器在低和高相对湿度下收集和保持埃博拉病毒感染力的能力。相对湿度不影响物理收集效率,但对于大多数采样器而言,在较高的相对湿度下,传染性得以更好地保留。全面的,基于25毫米明胶过滤器的物理和生物学效率,易用性,缺乏玻璃成分以及小批量回收以产生更浓缩的最终样品的能力,它们被认为是最佳的采样器。测试表明,与其他已发表方法相比,在2.5 mL培养基中回收并通过微量滴定使用埃博拉报道细胞的25 mm明胶过滤器可导致灵敏度提高约10倍。这项研究提供的数据将有助于促进利用雾化埃博拉病毒的不同研究之间的比较,并为将来的研究提供信息。测试表明,与其他已发表方法相比,在2.5 mL培养基中回收并通过微量滴定使用埃博拉报道细胞的25 mm明胶过滤器可导致灵敏度提高约10倍。这项研究提供的数据将有助于促进利用雾化埃博拉病毒的不同研究之间的比较,并为将来的研究提供信息。测试表明,与其他已发表方法相比,在2.5 mL培养基中回收并通过微量滴定使用埃博拉报道细胞的25 mm明胶过滤器可导致灵敏度提高约10倍。这项研究提供的数据将有助于促进使用雾化埃博拉病毒的不同研究之间的比较,并为将来的研究提供信息。

更新日期:2021-03-01
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