Borage oil [BO: 40.9% linoleic acid (LNA) and 24.0% γ‐linolenic acid (GLA)] reverses disrupted epidermal lipid barrier in essential fatty acid deficiency (EFAD). We determined the effects of BO on lamellar body (LB) content and LNA and GLA incorporation into epidermal ceramide 1 (CER1) and epidermal ceramide 2 (CER2), major barrier lipids. EFAD was induced in guinea pigs by a diet of 6% hydrogenated coconut oil (HCO) for 10 weeks (group HCO) or 8 weeks followed by 6% BO for 2 weeks (group HCO + BO). LB content and LNA and GLA incorporation into CER1 were higher in group HCO + BO than in group HCO. Small but significant levels of LNA, GLA, and their C20‐metabolized fatty acids [dihomo‐γ‐linolenic acid (DGLA) and arachidonic acid (ARA)] were incorporated into CER2, where ARA was detected at a level lower than LNA, but DGLA incorporation exceeded that for GLA in group HCO + BO. Dietary BO enhanced LB content and differential incorporation of GLA into CER1 and DGLA into CER2.

中文翻译：

---

琉璃苣油提高必需脂肪酸缺乏豚鼠表皮神经酰胺的层状体含量并改变脂肪酸组成

琉璃苣油 [BO：40.9% 亚油酸 (LNA) 和 24.0% γ-亚麻酸 (GLA)] 可逆转必需脂肪酸缺乏症 (EFAD) 中破坏的表皮脂质屏障。我们确定了 BO 对层状体 (LB) 含量以及 LNA 和 GLA 掺入表皮神经酰胺 1 (CER1) 和表皮神经酰胺 2 (CER2)（主要屏障脂质）的影响。EFAD 通过 6% 氢化椰子油 (HCO) 饮食 10 周（HCO 组）或 8 周，然后 6% BO 饮食 2 周（HCO + BO 组）在豚鼠中诱导。HCO+BO组的LB含量和LNA和GLA掺入CER1中高于HCO组。少量但显着的 LNA、GLA 及其 C20 代谢脂肪酸 [二高-γ-亚麻酸 (DGLA) 和花生四烯酸 (ARA)] 被纳入 CER2，其中检测到的 ARA 水平低于 LNA，但在 HCO + BO 组中，DGLA 的掺入超过了 GLA。膳食 BO 提高了 LB 含量和 GLA 进入 CER1 和 DGLA 进入 CER2 的差异掺入。