Aquaculture Reports ( IF 3.7 ) Pub Date : 2020-12-31 , DOI: 10.1016/j.aqrep.2020.100567 Nesreen A. Mohamed , Michel F. Saad , Mustafa Shukry , Abeer M.S. El-Keredy , Omaima Nasif , Hien Van Doan , Mahmoud A.O. Dawood
Nile tilapia (Oreochromis niloticus) is a well-known economic fish species and can grow well under optimum environmental conditions. However, stressful rearing conditions are expected to deteriorate the performance and health condition of fish. Hence, the present study investigated the physiological and ion changes of Nile tilapia under salinity stress. Fish of 200 ± 20 g were divided into three groups where the control group reared in water with 0 ppt salinity, while the second and third groups were raised in water with low salinity (10 ppt) and high salinity (15 ppt), respectively. Blood dissolved oxygen and gases (pH, PO2, PCO2, TCO2, and HCO3−) significantly differed in different groups after 5 and 10 days (P < 0.05). The salinity stress affected the Hb, PCV, Na+, K+, Ca++, and lactate significantly (P < 0.05). Serum cortisol, triiodothyronine, and free triiodothyronine concentrations after 5 and 10 days were significantly affected (P < 0.05) in salinity stressed groups compared to the control group. Serum malondialdehyde showed a significant increase (P < 0.05) after 5 days in the stressed groups, then became non-significant after 10 days compared to the control group. The relative gene expression of Na+-K+-ATPase and cytosolic carbonic anhydrase were significantly upregulated in salinity stressed groups (P < 0.05). The histopathological images of Nile tilapia exposed to high salinity stress revealed damage in gills, liver, and kidney tissues, which confirmed the harmful effects of increased salinity. Thus, the obtained results demonstrated that the adverse effects of high salinity stress on the health status of Nile tilapia.
中文翻译:
尼罗罗非鱼(生理离子变化尼罗罗非鱼盐度胁迫的作用下)
尼罗罗非鱼(Oreochromis niloticus)是一种著名的经济鱼类,可以在最佳环境条件下生长良好。但是,紧张的饲养条件预计会恶化鱼类的性能和健康状况。因此,本研究调查了盐度胁迫下尼罗罗非鱼的生理和离子变化。将200±20 g的鱼分为三组,对照组在盐度为0 ppt的水中饲养,而第二组和第三组分别在低盐度(10 ppt)和高盐度(15 ppt)的水中饲养。血液溶解氧和气体(pH值,PO 2,PCO 2, TCO 2和HCO 3 -)在5天和10天后在不同组中有显着差异(P <0.05)。盐分胁迫显着影响Hb,PCV,Na +,K +,Ca ++和乳酸(P <0.05)。与对照组相比,盐度胁迫组的血清皮质醇,三碘甲状腺素和游离三碘甲状腺素浓度在第5天和第10天受到显着影响(P <0.05)。与对照组相比,应激组中的血清丙二醛在5天后显示出显着增加(P <0.05),然后在10天后变得无显着性。Na + -K +的相对基因表达盐胁迫组的-ATPase和胞质碳酸酐酶显着上调(P <0.05)。暴露于高盐度胁迫下的尼罗罗非鱼的组织病理学图像显示in,肝和肾组织受到损害,这证实了盐度增加的有害影响。因此,获得的结果表明高盐度胁迫对尼罗罗非鱼健康状况的不利影响。