Parkinson’s disease (PD) is the second most neurodegenerative disease, which affects many persons worldwide. Tomentosin is an active compound isolated from I.viscosa plant that has extensive therapeutic values. In this exploration, the neuroprotective actions of tomentosin were investigated against the MPTP-stimulated neuroinflammation in mice. The PD was stimulated to the C57/BL6 mice by injecting the 20mg/kg of MPTP for 4 times with a 2h interval for 15 days along with the tomentosin treatment. The Rota-rod test, grasping test, and pole climbing test was executed to investigate the motor functions of test animals. The statuses of pro-inflammatory cytokines, reactive oxygen species, and myeloperoxidase were examined by using the commercial ELISA kits. The superoxide dismutase enzyme level was inspected by the standard method. The expression level of TLR-4/NF-ƙB was examined by western blotting analysis. The brain tissues of investigational animals were analyzed microscopically. The tomentosin treatment to the MPTP-intoxicated PD mice was displayed an appreciable regain in the bodyweight. Tomentosin notably prevented the MPTP-stimulated impairments in the motor functions. The status of pro-inflammatory cytokines, ROS, and MPO was assuaged by the tomentosin treatment in the brain tissues of PD mice. The tomentosin inhibits the TLR-4/NF-ƙB signaling pathway and also prevented the inflammation-mediated neuronal cells damages in the brain tissues of PD animals. The reduced glial cells damages and usual ganglion layers were seen in the brain tissues of tomentosin supplemented PD mice. These findings were confirmed the neuroprotective properties of tomentosin against the MP

中文翻译：

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绒毛膜素减轻了MPTP诱发的帕金森氏病小鼠的行为缺陷和神经炎症反应

帕金森氏病（PD）是第二大神经退行性疾病，影响全世界许多人。绒毛膜素是从粘膜肠杆菌植物中分离的活性化合物，具有广泛的治疗价值。在这项探索中，研究了绒毛膜毒素对小鼠MPTP刺激的神经炎症的神经保护作用。通过注射20mg / kg的MPTP 4次，间隔2h，持续15天，将PD刺激到C57 / BL6小鼠，同时进行tototosin处理。进行了Rota-rod测试，抓握测试和爬杆测试，以研究被测动物的运动功能。通过使用商用ELISA试剂盒检查促炎细胞因子，活性氧和髓过氧化物酶的状态。通过标准方法检查超氧化物歧化酶水平。通过蛋白质印迹分析检测TLR-4 /NF-κB的表达水平。用显微镜分析了被调查动物的脑组织。对MPTP中毒的PD小鼠进行tototosin处理后，体重得到了明显的恢复。tomentosin明显防止了MPTP刺激的运动功能障碍。在PD小鼠的脑组织中，通过tomentosin治疗可以减轻促炎性细胞因子，ROS和MPO的状态。tomentosin抑制TLR-4 /NF-κB信号通路，还防止了PD动物脑组织中炎症介导的神经元细胞的损伤。在补充了tomentosin的PD小鼠的脑组织中观察到神经胶质细胞损伤减少和通常的神经节层减少。这些发现证实了tomentosin对MP的神经保护作用