ABSTRACT

In this project, the CeO₂NP_s were synthesized using pollen grains of Brassica napus as a capping agent and reducing agent. The nanoceria were characterized by FTIR, FESEM, TEM, PXRD, and DLS. To increase the bioavailability, the nanoemulsion of cerium nanoparticle was prepared. The TEM images have revealed particles with spherical morphology and mean particle sizes of ca. 23.2±4.0 nm. The prepared sample after calcination at 500 °C showed the characteristic peaks of nanoceria with a good crystallinity. Chorioallantoic membrane (CAM) test results showed by increasing the concentration of biosynthesized CeO₂NP_s, the size and number of blood vessels were reduced. Also, the cytotoxicity test using MTT showed little toxicity of the biosynthetic CeO₂NP_s on the human foreskin fibroblast cell, whereas synthetic CeO₂NP_s destroyed ovarian cancer cells (A2780) which depends on the time and concentration of the NPs. The results of this research show excessive promise for drug treatments and pharmacological technology.

摘要

本项目以欧洲油菜花粉粒作为封端剂和还原剂合成了CeO ₂ NPs _。通过 FTIR、FESEM、TEM、PXRD 和 DLS 对纳米陶瓷进行了表征。为了提高生物利用度，制备了铈纳米颗粒的纳米乳液。TEM 图像揭示了具有球形形态和平均粒径约 10 的颗粒。23.2±4.0 纳米。所制备的样品在 500 ℃煅烧后呈现出纳米氧化铈的特征峰，具有良好的结晶度。尿囊膜 (CAM) 测试结果表明通过增加生物合成的 CeO ₂ NP _s的浓度，血管的大小和数量减少。此外，使用 MTT 进行的细胞毒性试验表明，生物合成的 CeO 2 NP 对人包皮成纤维细胞几乎没有毒性_，而合成的 CeO ₂ NP会破坏卵巢癌细胞（A2780），这取决于 NP 的时间和浓度_。这项研究的结果显示了对药物治疗和药理学技术的巨大希望。