2019 novel coronavirus (2019-nCoV) with strong contagion in the crowd, has ravaged worldwide and severely impacts the human health and epidemic prevention system, by producing a series of significant stress reactions in the body to induce further cytokine storm. Transcription factors (TFs) served as essential DNA binding proteins play an integral role in regulating cytokine storm, and the detection of it in the human coronavirus environment provides especially valuable approaches to diagnosis and treatment of 2019-nCoV and development of antiviral drugs. In this work, an entropy-driven electrochemiluminescence (ECL) biosensor was constructed for ultra-sensitive bioassay of NF-κB p50. The strategy primarily capitalizing the splendid double-stranded DNA (dsDNA) binding properties of transcription factors, employing GOAu-Ru composite material as ECL emitter, utilizing entropy-driven reactions for signal amplification method, offered a repeatable proposal for TFs detection. In the absence of TFs, the released DNA1 further went in the entropy-driven reaction, contributing to an “ECL off” state. However, in the presence of TFs, the dsDNA avoided being digested, which blocked DNA1 for participating in the entropy-driven reaction, and the system exhibited an “ECL on” state. Most importantly, the ECL bioanalytical method denoted broad application prospects for NF-κB p50 detection with a lower detection limit (9.1 pM).

2019新型冠状病毒（2019-nCoV）人群传染性强，肆虐全球，严重冲击人类健康防疫系统，在机体产生一系列显着的应激反应，引发进一步的细胞因子风暴。转录因子（TFs）作为必需的DNA结合蛋白在调节细胞因子风暴中发挥着不可或缺的作用，在人类冠状病毒环境中对其进行检测为2019-nCoV的诊断和治疗以及抗病毒药物的开发提供了特别有价值的方法。在这项工作中，构建了一个熵驱动的电化学发光 (ECL) 生物传感器，用于 NF-κB p50 的超灵敏生物测定。该策略主要利用转录因子出色的双链 DNA (dsDNA) 结合特性，采用 GOAu-Ru 复合材料作为 ECL 发射体，利用熵驱动反应进行信号放大方法，为 TFs 检测提供了可重复的建议。在没有转录因子的情况下，释放的 DNA1 进一步进入熵驱动反应，导致“ECL 关闭”状态。然而，在转录因子存在的情况下，dsDNA 避免被消化，从而阻断了 DNA1 参与熵驱动反应，系统呈现“ECL 开启”状态。最重要的是，ECL 生物分析方法表明 NF-κB p50 检测具有较低的检测限（9.1 pM）的广泛应用前景。促成“ECL 关闭”状态。然而，在转录因子存在的情况下，dsDNA 避免被消化，从而阻断了 DNA1 参与熵驱动反应，系统呈现“ECL 开启”状态。最重要的是，ECL 生物分析方法表明 NF-κB p50 检测具有较低的检测限（9.1 pM）的广泛应用前景。促成“ECL 关闭”状态。然而，在转录因子存在的情况下，dsDNA 避免被消化，从而阻断了 DNA1 参与熵驱动反应，系统呈现“ECL 开启”状态。最重要的是，ECL 生物分析方法表明 NF-κB p50 检测具有较低的检测限（9.1 pM）的广泛应用前景。