Chemical constituents from Zhideke granules were rapidly isolated and identified by ultra-performance liquid chromatography (UPLC) coupled with hybrid quadrupole-orbitrap mass spectrometry (MS) in positive and negative ion modes using both full scan and two-stage threshold-triggered mass modes. The secondary fragment ion information of the target compound was selected and compared with the compound reported in databases and related literatures to further confirm the possible compounds. A total of 47 chemical constituents were identified from the ethyl acetate extract of Zhideke granules, including 21 flavonoids and glycosides, 9 organic acids, 4 volatile components, 3 nitrogen-containing compounds, and 10 other compounds according to the fragmentation patterns, relevant literature, and MS data. The result provides a new method for the analysis of chemical constituents of Zhideke granules which laid the foundation for quality control and the study of pharmacodynamic materials of Zhideke granules.

中文翻译：

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高效液相色谱-质谱联用分离制止得克颗粒中的化学成分

使用全扫描和两阶段阈值触发质量模式，在正离子和负离子模式下，通过超高效液相色谱（UPLC）与混合四极杆-轨道阱质谱（MS）结合，快速分离和鉴定智德科颗粒的化学成分。选择目标化合物的次级碎片离子信息，并将其与数据库和相关文献中报道的化合物进行比较，以进一步确认可能的化合物。根据碎裂模式，从智德科颗粒的乙酸乙酯提取物中共鉴定出47种化学成分，包括21种类黄酮和糖苷，9种有机酸，4种挥发性成分，3种含氮化合物和10种其他化合物，相关文献，和MS数据。