NF-Y is a trimeric Transcription Factor -TF- which binds with high selectivity to the conserved CCAAT element. Individual ChIP-seq analysis as well as ENCODE have progressively identified locations shared by other TFs. Here, we have analyzed data introduced by ENCODE over the last five years in K562, HeLa-S3 and GM12878, including several chromatin features, as well RNA-seq profiling of HeLa-S3 cells after NF-Y inactivation. We double the number of sequence-specific TFs and co-factors reported. We catalogue them in 4 classes based on co-association criteria, infer target genes categorizations, identify positional bias of binding sites and gene expression changes. Larger and novel co-associations emerge, specifically concerning subunits of repressive complexes as well as RNA-binding proteins. On the one hand, these data better define NF-Y association with single members of major classes of TFs, on the other, they suggest that it might have a wider role in the control of mRNA production.

NF-Y是三聚体转录因子-TF-，其与保守的CCAAT元件高选择性结合。单独的ChIP-seq分析以及ENCODE已逐步确定了其他TF共享的位置。在这里，我们分析了ENCODE在过去的五年中在K562，HeLa-S3和GM12878中引入的数据，包括一些染色质特征，以及NF-Y失活后HeLa-S3细胞的RNA序列分析。我们将报道的序列特异性TF和辅助因子的数量增加了一倍。我们基于共同关联标准将它们分为4类，推断目标基因的分类，识别结合位点的位置偏差和基因表达的变化。出现了更大且新颖的共缔合，特别是关于抑制复合物的亚基以及RNA结合蛋白。一方面，