Yarrowia is a yeast genus that has been used as a model oleaginous taxon for a wide array of studies. However, information regarding metabolite changes within Yarrowia spp. under different environmental conditions is still limited. Among various factors affecting Yarrowia metabolism, nitrogen-limiting conditions have a profound effect on the metabolic state of yeast. In this study, a time-course LC-MS/MS-based metabolome analysis of Y. lipolytica was performed to determine the optimal cultivation time and carbon-to-nitrogen ratio for studying the effects of nitrogen-limiting conditions on Yarrowia; we found that cultivation time of 36 h and carbon-to-nitrogen ratio of 4:1 and 5:0 was suitable for studying the effects of nitrogen-limiting conditions on Yarrowia and these conditions were applied to six strains of Yarrowia. These six strains of Yarrowia showed similar responses to nitrogen-limiting conditions; however, each strain had a unique metabolomic profile. Purine and pyrimidine metabolism were the most highly affected biological pathways in nitrogen-limiting conditions, indicating that these conditions affect energy availability within cells. This stress leads to a shift in cells to the utilization of a less ATP-dependent biological pathway. This information will be beneficial for the development of Yarrowia strains for further scientific and industrial applications.

中文翻译：

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耶氏酵母属对氮限制条件响应的代谢组学分析。

耶氏酵母属（Yarrowia）是酵母属，已被用作模型油质分类群，用于许多研究。但是，有关耶氏酵母属中代谢物变化的信息。在不同的环境条件下仍然受到限制。在影响耶氏酵母属代谢的各种因素中，氮限制条件对酵母的代谢状态具有深远的影响。在这项研究中，进行了基于时程LC-MS / MS的解脂耶氏酵母代谢组分析，以确定最佳的培养时间和碳氮比，以研究限氮条件对耶氏酵母的影响。; 我们发现，培养时间为36小时，碳氮比为4：1和5：0适于研究限氮条件对耶氏酵母的影响，并将这些条件应用于六株耶氏酵母。这六种耶氏耶氏酵母菌株显示出对氮限制条件的相似反应。但是，每种菌株都有独特的代谢组学特征。在氮限制条件下，嘌呤和嘧啶代谢是受影响最大的生物途径，表明这些条件影响细胞内的能量利用。这种压力导致细胞转移到利用较少的ATP依赖性生物途径。这些信息将有助于耶氏酵母的发展 菌株用于进一步的科学和工业应用。