ABSTRACT

Human adipose-derived stem cells (hADSCs) are adult mesenchymal cells that have attracted the interest of clinical scientists and surgeons due to their large number of advantages including ease of access and expansion, abundance in cell culture, high proliferative rates, and lower senescence. SUMO/sentrin specific protease 1 (SENP1) is a critical protease that is required during the process of SUMOylation and deSUMOylation, which are dynamic mechanisms that influence cell cycle progression, cell proliferation, and apoptotic status. However, the contribution of SENP1 to these important cellular processes in hADSCs is largely uncharacterized and further studies in this area are required. Here, we show for the first time that after knock out SENP1 in hADSCs, their capacity to migrate and proliferate were inhibited, while apoptosis was enhanced. However, SENP1 did not significantly influence the morphology and MSC-related phenotypes of the hADSCs. These results highlight a role for SENP1 during hADSC growth, and its potential as a therapeutic target to improve the efficacy and safety of hADSCs in the clinic.

摘要

人类脂肪干细胞 (hADSCs) 是一种成人间充质细胞，由于其众多优势，包括易于获取和扩增、细胞培养丰富、增殖率高和衰老率低，引起了临床科学家和外科医生的兴趣。SUMO/sentrin 特异性蛋白酶 1 (SENP1) 是 SUMOylation 和 deSUMOylation 过程中所需的一种关键蛋白酶，这是影响细胞周期进程、细胞增殖和凋亡状态的动态机制。然而，SENP1 对 hADSCs 中这些重要细胞过程的贡献在很大程度上是未知的，需要在该领域进一步研究。在这里，我们首次表明在 hADSCs 中敲除 SENP1 后，它们的迁移和增殖能力受到抑制，而细胞凋亡增强。然而，SENP1 没有显着影响 hADSCs 的形态和 MSC 相关表型。这些结果突出了 SENP1 在 hADSC 生长过程中的作用，以及它作为提高 hADSCs 在临床中的疗效和安全性的治疗靶点的潜力。