Trachurus japonicus is an economically important fish in the northwestern Pacific Ocean. However, its resources have declined seriously and there is an urgent need for a wide‐range of investigations of the existing genetic resources. This requires a large number of diverse molecular markers with high discriminating power. In this study, we identified 43,264 perfect SSRs in T. japonicus genome using SLAF‐seq technology. Of these, we randomly selected 106 SSRs (tri‐nucleotide to hexa‐nucleotide) to test for polymorphism. Eventually, we successfully developed a total of 33 loci including 8 tri‐nucleotide and 25 long repeat motifs (tetra‐nucleotide to hexa‐nucleotide). The number of alleles (Na) of these loci ranged from 4 to 24 (mean 12.6). The observed heterozygosity (Ho) and expected heterozygosity (He) varied from 0.258 to 0.969 (mean 0.723) and from 0.452 to 0.962 (mean 0.827), respectively. All loci except TJ6‐7 were highly informative (PIC > 0.5). These results showed that the shortlisted 33 loci exhibited moderate to relatively high genetic diversity, of which 18 were regarded as highly polymorphic and well‐resolved. In summary, these diverse and potential microsatellites detected in our study provide substantial genetic basis for the screening of polymorphic SSR markers of T. japonicus and also provide a powerful tool to perform further studies on the genetic resource assessment and conservation of T. japonicus.

中文翻译：

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利用SLAF-seq技术开发和鉴定日本稻草中33个多态性微卫星标记

Trachurus japonicus是西北太平洋中一种重要的经济鱼类。但是，其资源已严重减少，迫切需要对现有遗传资源进行广泛的调查。这需要具有高辨别力的大量多样的分子标记。在这项研究中，我们使用SLAF-seq技术鉴定了T. japonicus基因组中的43,264个完美SSR 。其中，我们随机选择了106个SSR（三核苷酸到六核苷酸）以测试多态性。最终，我们成功地开发了总共33个基因座，包括8个三核苷酸和25个长重复基序（四核苷酸到六核苷酸）。这些基因座的等位基因（Na）数量范围为4至24（平均12.6）。观察到的杂合性（Ho）和预期杂合度（He）分别从0.258到0.969（平均0.723）和0.452到0.962（平均0.827）。除TJ6-7以外的所有基因座都具有很高的信息含量（PIC  > 0.5）。这些结果表明，入围的33个基因座表现出中等至相对较高的遗传多样性，其中18个被认为是高度多态的，并且得到了很好的解析。综上所述，在我们的研究中检测到的这些多样且潜在的微卫星不仅为筛选日本血吸虫的多态性SSR标记提供了重要的遗传基础，而且为进行进一步的研究对日本血吸虫的遗传资源进行评估和保护提供了有力的工具。