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Rhubarb anthraquinone glycosides protect against cerebral ischemia‐reperfusion injury in rats by regulating brain–gut neurotransmitters
Biomedical Chromatography ( IF 1.8 ) Pub Date : 2020-12-29 , DOI: 10.1002/bmc.5058
Ying Guo 1 , Qiuying Li 1 , Xiahui Yu 1 , Yuhua Liang 1
Affiliation  

Rhubarb anthraquinone glycosides (RAGs) have been proven to have significant therapeutic effects on ischemic stroke, and this effect may be related to the microbiome–gut–brain axis. In this study, an HPLC‐FLD method was established to measure brain–gut neurotransmitters of rats with cerebral ischemia‐reperfusion injury (CIRI), to explore whether the mechanism of RAGs against CIRI is related to the microbiome–gut–brain axis. A Shimadzu ODS‐3 C18 column was used for chromatographic separation, and 5‐hydroxytryptamine (5‐HT), 5‐hydroxy indole acetic acid (5‐HIAA), glutamic acid (Glu), aspartic acid (Asp), and γ‐aminobutyric acid (GABA) were determined simultaneously. The results showed that there is an excellent linear relationship (R2≥ 0.9990) and a high separation degree in the HPLC‐FLD method. Whereas the contents of Asp and Glu in the brain and colon increased (p < 0.05), the contents of 5‐HT, 5‐HIAA, and GABA in the brain and colon decreased (p < 0.05) after CIRI. RAGs could effectively reduce the contents of Asp and Glu (p < 0.05), and increase the contents of 5‐HT, 5‐HIAA, and GABA in the brain and colon (p < 0.05). Combined with the previous experimental results, we can speculate that RAGs can regulate intestinal flora disorder caused by CIRI, and then regulate the imbalance between the release and decomposition of neurotransmitters caused by intestinal flora disorder.

中文翻译:

大黄蒽醌糖苷通过调节脑肠神经递质来预防大鼠脑缺血再灌注损伤

大黄蒽醌糖苷(RAGs)已被证明对缺血性中风具有显着的治疗作用,这种作用可能与微生物组-肠-脑轴有关。在这项研究中,建立了一种HPLC-FLD方法来测量患有脑缺血再灌注损伤(CIRI)的大鼠的脑肠神经递质,以探讨RAGs对抗CIRI的机制是否与微生物组-肠-脑轴相关。使用Shimadzu ODS-3 C 18色谱柱进行色谱分离,并使用5-羟基色胺(5-HT),5-羟基吲哚乙酸(5-HIAA),谷氨酸(Glu),天冬氨酸(Asp)和γ同时测定了氨基丁酸(GABA)。结果表明,存在良好的线性关系(R 2≥0.9990)和HPLC-FLD方法的高分离度。CIRI后,脑和结肠中Asp和Glu的含量增加(p <0.05),而脑和结肠中5-HT,5-HIAA和GABA的含量降低(p <0.05)。RAGs可以有效地降低脑和结肠中Asp和Glu的含量(p <0.05),并增加脑内和结肠中5-HT,5-HIAA和GABA的含量(p <0.05)。结合以前的实验结果,我们可以推测RAGs可以调节由CIRI引起的肠道菌群失调,然后调节由肠道菌群失调引起的神经递质的释放和分解之间的不平衡。
更新日期:2020-12-29
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