当前位置: X-MOL 学术Exp. Eye Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
circRNA-miRNA-mRNA network in age-related macular degeneration: From construction to identification
Experimental Eye Research ( IF 3.4 ) Pub Date : 2020-12-29 , DOI: 10.1016/j.exer.2020.108427
Yu Su , Yuexiong Yi , Lu Li , Changzheng Chen

The aim of the present study was to investigate the pathogenesis of age-related macular degeneration (AMD) by constructing a regulatory circRNA-miRNA-mRNA network. By adjusting the P value to <0.05 and the absolute log value of fold change to >0.25, 2920 and 1057 differentially expressed mRNAs were identified from GSE50195 and GSE29801, respectively. Based on a literature review, Starbase database analysis, and RNA hybrid assays, we obtained 77 miRNA-mRNA and 331 circRNA-miRNA pairs. After combining these pairs, we constructed a circRNA-miRNA-mRNA network possessing 303 circRNA nodes, 4 miRNA nodes, 51 mRNA nodes, and 408 edges. By utilizing protein-protein network analysis, the MCODE algorithm, and the highest degree of circRNA node, we identified the regulatory axis of hsa_circRNA7329/hsa-miR-9/SCD. Hsa_circRNA7329 may regulate SCD through hsa-miR-9 to promote macrophage-mediated inflammation and pathologic angiogenesis, which lead to AMD development. However, the underlying details require further investigation.



中文翻译:

circRNA-miRNA-mRNA网络与年龄相关性黄斑变性:从构建到鉴定

本研究的目的是通过构建调节性circRNA-miRNA-mRNA网络来研究年龄相关性黄斑变性(AMD)的发病机制。通过将P值调整为<0.05,将倍数变化的绝对对数值调整为> 0.25,分别从GSE50195和GSE29801鉴定了2920和1057差异表达的mRNA。基于文献综述,Starbase数据库分析和RNA杂交测定,我们获得了77对miRNA-mRNA和331对circRNA-miRNA。结合这些对后,我们构建了一个具有303个circRNA节点,4个miRNA节点,51个mRNA节点和408个边缘的circRNA-miRNA-mRNA网络。通过利用蛋白质网络分析,MCODE算法和最高程度的circRNA节点,我们确定了hsa_circRNA7329 / hsa-miR-9 /的调控轴SCD。Hsa_circRNA7329可能通过hsa-miR-9调节SCD,从而促进巨噬细胞介导的炎症和病理性血管生成,从而导致AMD的发展。但是,底层细节需要进一步调查。

更新日期:2021-01-06
down
wechat
bug