Artificial gravity is a potential countermeasure to attenuate effects of weightlessness during long-term spaceflight, including losses of muscle mass and function, possibly to some extent attributable to disturbed neuromuscular interaction. The 60-day AGBRESA bed-rest study was conducted with 24 participants (16 men, 8 women; 33 ± 9 years; 175 ± 9 cm; 74 ± 10 kg; 8 control group, 8 continuous (cAG) and 8 intermittent (iAG) centrifugation) to assess the impact of bed rest with or without daily 30-min continuous/intermittent centrifugation with 1G at the centre of mass. Fasting blood samples were collected before and on day 6, 20, 40 and 57 during 6° head-down tilt bed rest. Concentrations of circulating markers of muscle wasting (GDF-8/myostatin; slow skeletal muscle troponin T; prostaglandin E2), neurotrophic factors (BDNF; GDNF) and C-terminal Agrin Fragment (CAF) were determined by ELISAs. Creatine kinase activity was assessed by colorimetric enzyme assay. Repeated-measures ANOVAs were conducted with TIME as within-subject, and INTERVENTION and SEX as between-subject factors. The analyses revealed no significant effect of bed rest or sex on any of the parameters. Continuous or intermittent artificial gravity is a safe intervention that does not have a negative impact of the neuromuscular secretome.

人工重力是减轻长期航天过程中失重影响的潜在对策，包括肌肉质量和功能的丧失，可能在某种程度上归因于神经肌肉相互作用的紊乱。为期60天的AGBRESA卧床研究是针对24名参与者（16名男性，8名女性； 33±9岁； 175±9厘米； 74±10公斤； 8个对照组，8个连续（cAG）和8个间歇性（iAG） ）离心）以评估在有或没有每日30分钟连续/间歇离心，质心为1G的情况下卧床休息的影响。在6°头朝下倾斜卧床休息期间，在第6、20、40和57天之前和第6天以及第6天，采集空腹血液样本。肌肉消瘦的循环标志物（GDF-8 /肌生长抑制素；慢速骨骼肌肌钙蛋白T；前列腺素E2），神经营养因子（BDNF； NDF）的浓度。通过ELISA确定GDNF和C末端Agrin片段（CAF）。通过比色酶测定法评估肌酸激酶活性。重复测量方差分析以时间为对象内，时间和性别为对象间因素。分析表明，卧床休息或性别对任何参数均无明显影响。连续或间歇性人工重力是一种安全的干预措施，不会对神经肌肉分泌组产生负面影响。