Crown gall disease caused by Agrobacterium tumefaciens causes economic loss in ornamental production in Japan. To breed a resistant rootstock, we used crown gall resistant Rosa ‘PEKcougel’ to hybridized with tetraploid of R. multiflora ‘Matsushima No. 3’. In this study, we investigated the crown gall resistance characteristics in their progenies. In the needle prick test, eight tested progenies showed significantly smaller wounds than R. ‘PEKcougel’. In the stem segment culture and opine assay, six progenies formed no callus tissue while three progenies formed calluses and had no opine detected. Further oncogene expression analysis was performed to evaluate crown gall disease resistance, since the low callus formation rate limited the application of opine assay. In the oncogene expression analysis, some progenies of tetraploid of R. multiflora ‘Matsushima No. 3’ showed a lower expression level of both ipt and iaaM than R. ‘PEKcougel’. Furthermore, ipt expression had a significant positive relationship with tumor size. Taken together, these results suggest that ipt expression analysis can be used for evaluating crown gall disease resistance in rose progenies. Furthermore, eight progenies with strong crown gall disease resistance were confirmed.

由根癌农杆菌引起的冠gall病在日本的观赏生产中造成经济损失。为了育种抗性砧木，我们使用了抗冠gall的罗莎“ PEKcougel”与多花红景天“ Matsushima No.3”的四倍体杂交。在这项研究中，我们调查了后代的冠gall抗性特征。在针刺试验中，八个被测后代显示出比R小得多的伤口。 'PEKcougel'。在茎段培养和阿片样物质测定中，六个子代没有形成愈伤组织，而三个子代形成了愈伤组织并且没有检测到阿片。由于愈伤组织形成率低，限制了阿片类药物的应用，因此进行了进一步的癌基因表达分析，以评估冠crown病的抗药性。在癌基因表达分析，四倍体的一些后代野蔷薇“第3号松”表明两者的较低表达水平IPT和的iaaM比R.  “PEKcougel”。此外，ipt表达与肿瘤大小有显着正相关。综上所述，这些结果表明ipt表达分析可用于评估玫瑰后代的冠crown病抗性。此外，证实了八种具有强冠crown病抗性的后代。