In this paper, ultrasonic-assisted deep eutectic solvent (DES) extraction was applied to the acquisition of chromones (cimicifugin, prim-o-glucosylcimifugin, and 5-o-methylvisamminoside) from Saposhnikoviae radix (SR). The extraction effects of 11 prepared DESs were screened taking contents of chromones as indexes. Furthermore, the optimum extraction conditions were confirmed using a single-factor test and response surface optimization test. Scavenging activities of DPPH anion and ABTS cation radicals of different SR extracts (DES, methanol, and ethanol) were studied. The analysis results of best extraction conditions optimized by Design-Expert software were as follows: extraction time (40 min), extraction temperature (60°C), and the solid/liquid ratio (32 mL/g). Scavenging rates of the DES extract for DPPH anion radical and ABTS cation radical were found to be 75.31% and 65.71%, which were higher than those of methanol and ethanol extracts. In conclusion, the developed extraction method can be regarded as a safe, green, and more effective approach for the extraction of chromones in SR.

中文翻译：

---

超声辅助深共晶溶剂萃取获得的沙波氏杆菌中的醌类抗氧化能力分析

在本文中，超声辅助的深共晶溶剂（DES）萃取施加到采集色酮类（cimicifugin，升麻邻苷，和5-O-methylvisamminoside）从防风基数（SR）。以色酮含量为指标，筛选了11种制备的DES的提取效果。此外，使用单因素试验和响应面优化试验确定了最佳提取条件。研究了不同SR提取物（DES，甲醇和乙醇）中DPPH阴离子和ABTS阳离子的清除活性。通过Design-Expert软件优化的最佳萃取条件的分析结果如下：萃取时间（40分钟），萃取温度（60°C）和固/液比（32 mL / g）。DES提取物对DPPH阴离子自由基和ABTS阳离子自由基的清除率分别为75.31％和65.71％，高于甲醇和乙醇提取物。总之，开发的提取方法可以被视为安全，绿色，