ABSTRACT

In the current study, silver nanoparticle (AgNPs) is biogenically synthesised using marine algae Cladophora glomerata extract, and anticancer activity was studied against colon cancer cells HCT-116 in-vitro. Biosynthesised silver nanoparticles characterised using TEM, UV-Spectrophotometry, XRD, and FTIR. XRD and UV-Spectrophotometry confirmed AgNPs formation with a mean size of 8–11 nm. AgNPs demonstrated specific cytotoxicity to colon cancer cells, whereas no or minimal effect was observed in human colon epithelial cell line cells. The fluorescence microscopic of AgNPs treated HCT-116 after nucleus staining confirmed the apoptosis induction in HCT-116 cells. In the immunoblotting study, the expression of proapoptotic markers like caspases, p21, BAX/BCL-XL, and NF-kB, along with a decrease of further confirmed the p53-dependent apoptosis induced by AgNPs. Hence, overall, we concluded that our biogenically synthesised AgNPs nanoparticles possess a selective apoptotic effect against colon cancer HCT-116 cells and potential to be used in targeted therapy for colon cancer.

摘要

在目前的研究中，银纳米颗粒 (AgNPs) 是使用海藻 Cladophora glomerata生物合成的。 提取物，并在体外研究了对结肠癌细胞 HCT-116 的抗癌活性。使用 TEM、UV 分光光度法、XRD 和 FTIR 表征生物合成的银纳米颗粒。XRD 和紫外分光光度法证实了平均尺寸为 8-11 nm 的 AgNPs 形成。AgNPs 表现出对结肠癌细胞的特异性细胞毒性，而在人结肠上皮细胞系细胞中未观察到影响或影响很小。细胞核染色后，AgNPs 处理 HCT-116 的荧光显微镜证实了 HCT-116 细胞的凋亡诱导。在免疫印迹研究中，促凋亡标志物如半胱天冬酶、p21、BAX/BCL-XL 和 NF-kB 的表达以及减少进一步证实了 AgNPs 诱导的 p53 依赖性细胞凋亡。因此，总体而言，