Knowing the regional lineages/sublineages of human papillomavirus 31 (HPV 31) and 45 would be of great importance for further evolutionary, epidemiological, and biological analysis. In this regard, to characterize more common lineages and sublineages of HPV 31 and 45, the sequence variations of E6 gene were investigated in normal, premalignant, and malignant samples collected from the cervix in Iran. In total, 54 HPV 31‐ and 24 HPV 45‐positive samples were analyzed by hemi‐nested polymerase chain reaction (PCR) and nested‐PCR, respectively. All PCR products were subjected to direct sequencing analysis. The results indicated that all three lineages A, B, and C were detected in HPV 31‐positive samples; among which HPV 31 lineage A was dominant as it was found in 66.7% of all samples. HPV 31 lineages B and C were identified in 5.5% and 27.8% of samples, respectively. In HPV 45‐infected samples, lineage B comprised of 62.5% of all samples and the remaining 37.5% belonged to lineage A. In conclusion, our findings showed that lineage A of HPV 31 was predominant in Iran. Lineage B of HPV 45 was also dominant among Iranian women. However, further studies with larger sample size should be addressed to estimate the pathogenicity risk of HPV 31 or HPV 45 lineages/sublineages in the development of cervical cancer among Iranian women.

中文翻译：

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伊朗妇女宫颈样本中人乳头瘤病毒 31 型和 45 型的谱系分析

了解人乳头瘤病毒 31 (HPV 31) 和 45 的区域谱系/亚谱系对于进一步的进化、流行病学和生物学分析非常重要。在这方面，为了表征 HPV 31 和 45 的更常见谱系和亚谱系，在从伊朗子宫颈收集的正常、癌前和恶性样本中研究了 E6 基因的序列变异。总共，分别通过半巢式聚合酶链反应 (PCR) 和巢式 PCR 分析了 54 个 HPV 31 和 24 个 HPV 45 阳性样本。所有PCR产物均进行直接测序分析。结果表明，在 HPV 31 阳性样本中检测到了所有 A、B 和 C 三个谱系；其中HPV 31 A谱系占主导地位，占所有样本的66.7%。分别在 5.5% 和 27.8% 的样本中鉴定出 HPV 31 谱系 B 和 C。在 HPV 45 感染样本中，B 系占所有样本的 62.5%，其余 37.5% 属于 A 系。总之，我们的研究结果表明 HPV 31 的 A 系在伊朗占主导地位。HPV 45 谱系 B 在伊朗女性中也占主导地位。然而，应进行更大样本量的进一步研究，以估计 HPV 31 或 HPV 45 谱系/亚谱系在伊朗女性患宫颈癌中的致病风险。