A novel Bacillus sp.PM06 isolated from sugarcane waste pressmud was tested for extracellular α-amylase and cellulase enzyme production. The effect of different substrates, nitrogen sources, pH, and temperature on growth and extracellular enzyme production was examined. Bacillus sp.PM06 was able to grow with starch and carboxymethyl cellulose (CMC) as a sole source of carbon and ammonium chloride was found to be the best nitrogen source. Maximum enzyme production was obtained at 48 H for both α-amylase and cellulase. The optimal condition for measuring enzyme activity was found to be pH 5.5 at 50 °C for α-amylase and pH 6.4 at 60 °C for cellulase respectively. It was found that Bacillus sp.PM06 exhibited halotolerance up to 2 M Sodium chloride (NaCl) and Potassium chloride (KCl). The isolate could produce α-amylase in the presence of 2 M NaCl and 1 M KCl. However, the strain produced cellulase even in the presence of 2 M NaCl and KCl. Concomitant production of both enzymes was observed when the medium was supplemented with starch and CMC. A maximum of 31 ± 1.15 U/mL of amylase and 15 ± 1.5 U/mL of cellulase was produced in 48 H. The enzyme was partially purified by Ammonium sulphate (NH₄)₂SO₄ precipitation with 2.2 and 2.3-fold purification.

中文翻译：

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从甘蔗压泥中分离的新型耐盐芽孢杆菌 PM06 生产 α-淀粉酶和纤维素酶

测试了从甘蔗废榨泥中分离的一种新型芽孢杆菌属sp.PM06 的细胞外 α-淀粉酶和纤维素酶的产生。检查了不同底物、氮源、pH 和温度对生长和细胞外酶产生的影响。Bacillus sp.PM06 能够以淀粉和羧甲基纤维素 (CMC) 作为唯一的碳源生长，并且发现氯化铵是最好的氮源。α-淀粉酶和纤维素酶均在 48 小时获得最大酶产量。发现测量酶活性的最佳条件是 α-淀粉酶在 50 °C 时的 pH 值 5.5 和纤维素酶在 60 °C 时的 pH 值 6.4。发现芽孢杆菌sp.PM06 表现出高达 2 M 氯化钠 (NaCl) 和氯化钾 (KCl) 的耐卤性。该分离物在 2 M NaCl 和 1 M KCl 存在下可产生 α-淀粉酶。然而，即使在 2 M NaCl 和 KCl 存在下，该菌株也能产生纤维素酶。当培养基添加淀粉和 CMC 时，观察到两种酶同时产生。在 48 小时内最多可产生 31 ± 1.15 U/mL 的淀粉酶和 15 ± 1.5 U/mL 的纤维素酶。该酶通过硫酸铵 (NH ₄ ) ₂ SO ₄沉淀进行部分纯化，纯化率分别为 2.2 和 2.3 倍。