We aimed to identify specific circular RNAs (circRNAs) involved in bone repair of trauma-induced osteonecrosis of femoral head (TIONFH) and to explore the potential mechanism. CircRNA sequencing on the blood sample collected from patients with and without TIONFH was performed to select cirRNAs that were significantly differentially expressed, followed by qRT-PCR confirmation. Furthermore, the functions of one selected circRNA and the potential mechanisms in bone repair of TIONFH were validated based on the bone marrow mesenchymal stem cells (BMSCs) and osteoclast-like cells (OLCs) through CCK-8, flow cytometry, transwell assay, luciferase reporter assay, and western blot. A total of 234 upregulated and 148 downregulated differentially expressed circRNAs were identified, and qRT-PCR showed that circRNA_25487 was significantly upregulated in the peripheral blood of TIONFH patients. Luciferase reporter assay confirmed the binding effect between miR-134-3p and circRNA_25487. CircRNA_25487 suppression and miR-134-3p overexpression could promote cell proliferation and invasion while inhibited apoptosis of BMSCs and OLCs. miR-134-3p could target p21. CircRNA_25487 inhibited bone repair in TIONFH by sponging miR-134-3p to upregulate the expression of p21.

我们旨在鉴定参与创伤性股骨头坏死（TIONFH）的骨修复的特定环状RNA（circRNA），并探讨其潜在机制。对有或没有TIONFH患者的血液样本进行CircRNA测序，以选择差异显着表达的cirRNA，然后进行qRT-PCR确认。此外，基于骨髓间充质干细胞（BMSCs）和破骨细胞样细胞（OLCs）通过CCK-8，流式细胞术，transwell测定，荧光素酶的验证，选择了一种circRNA的功能以及其修复TIONFH的潜在机制。报告基因测定和蛋白质印迹。总共鉴定了234个上调和148个下调的差异表达circRNA，qRT-PCR结果显示TIONFH患者外周血中circRNA_25487明显上调。萤光素酶报告基因测定证实了miR-134-3p与circRNA_25487之间的结合作用。CircRNA_25487抑制和miR-134-3p过表达可促进细胞增殖和侵袭，同时抑制BMSC和OLC的凋亡。miR-134-3p可以靶向p21。CircRNA_25487通过刺激miR-134-3p上调p21的表达来抑制TIONFH中的骨修复。