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Characterizing dynamic changes of plasma cell-free Echinococcus granulosus DNA before and after cystic echinococcosis treatment initiation
Genomics ( IF 4.4 ) Pub Date : 2020-12-28 , DOI: 10.1016/j.ygeno.2020.12.035
Yanping Zhao 1 , Quzhen Gongsang 2 , Jingkai Ji 1 , Junhua Li 3 , Fahai Qi 4 , Jiandong Li 1 , Gezhen Qiangba 1 , Wangmu Danzeng 5 , Fang Chen 6 , Hongcheng Zhou 7 , Huasang 5 , Jiefang Yin 1 , Na Pei 1 , Jiandan Xie 1 , Huimin Cai 1 , Asan 5 , Huasheng Pang 2 , Jingzhong Li 2 , Weijun Chen 8 , Bin Li 9
Affiliation  

Over one million people are living with cystic echinococcosis (CE) and alveolar echinococcosis (AE). For CE, long-term albendazole treatment is often needed, which requires regular follow-up. Follow-up is mainly through imaging which is insensitive to subtle changes and subjective to experience. We investigated the changes of Echinococcus granulosus (Eg) cell-free DNA (cfDNA) in plasma of CE patients before and after albendazole treatment to evaluate its potential as an objective marker for treatment follow-up. Plasma samples of nine CE patients were collected before and after treatment. We identified Eg cfDNA from every sample through high-throughput sequencing. Eg cfDNA concentration and fragment length increased significantly after the treatment period. Ultrasound examination before and after the treatment initiation reflected the drug effects to a certain extent, as the cyst size of four patients reduced. Our findings indicated that Eg cfDNA from plasma could be a potential marker in the monitoring of CE treatment.



中文翻译:

表征囊性包虫病治疗开始前后浆细胞游离细粒棘球绦虫 DNA 的动态变化

超过 100 万人患有囊性棘球蚴病 (CE) 和肺泡型棘球蚴病 (AE)。对于CE,通常需要长期阿苯达唑治疗,需要定期随访。随访主要是通过对细微变化不敏感、对体验主观感受的成像。我们研究了阿苯达唑治疗前后 CE 患者血浆中细粒棘球绦虫 (Eg)游离 DNA (cfDNA) 的变化,以评估其作为治疗随访客观标志物的潜力。在治疗前后收集了 9 名 CE 患者的血浆样本。我们通过高通量测序从每个样本中鉴定出Eg cfDNA。例如cfDNA 浓度和片段长度在治疗期后显着增加。治疗开始前后的超声检查在一定程度上反映了药物作用,4例患者的囊肿缩小。我们的研究结果表明,来自血浆的Eg cfDNA 可能是监测 CE 治疗的潜在标志物。

更新日期:2021-01-28
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