Adiponectin is an important hormone that regulates systemic metabolism, and it has been reported that globular adiponectin promotes myogenic differentiation. However, the mechanisms by which adiponectin promotes myogenic differentiation is not fully understood. In the present study, we show that adiponectin and its receptor 1 are significantly up-regulated during myogenic differentiation and that adiponectin increased the expression level of a core myogenic regulator, Mef2C, which is required for the effects of adiponectin on promoting myogenic differentiation. A transcriptional inhibitor of Mef2C, HDAC9, was down-regulated by adiponectin. In turn, Mef2C overexpression up-regulates adiponectin and its receptor, AdipoR1, to increase myogenic differentiation. We showed that mechanistically, Mef2C directly binds to AdipoR1 promoter to transcriptionally up-regulate AdipoR1 expression, which is required for the effects of Mef2C overexpression on myogenic differentiation. Thus, adiponectin/AdipoR1 and Mef2c form a positive feedback loop to promote myogenic differentiation.

脂联素是调节全身代谢的重要激素，并且已经报道球状脂联素促进肌原性分化。但是，脂联素促进肌原性分化的机制尚未完全了解。在本研究中，我们显示脂联素及其受体1在成肌分化过程中显着上调，脂联素增加了核心成肌调节剂Mef2C的表达水平，这是脂联素促进成肌分化的作用所必需的。Mef2C的转录抑制剂HDAC9被脂联素下调。反过来，Mef2C过表达上调脂联素及其受体AdipoR1，以增加成肌分化。我们从机械上证明了这一点，Mef2C直接与AdipoR1启动子结合以转录上调AdipoR1表达，这是Mef2C过表达对肌原性分化的影响所必需的。因此，脂联素/ AdipoR1和Mef2c形成正反馈回路，以促进成肌分化。