Planarians are freshwater flatworms commonly used as environmental bioindicator due to their sensitivity of response and their ease of culturing in lab. Nevertheless, to date, very few studies describing their behavior have been led. This work aims to fill the literature gap by providing preliminary results through six behavioral challenges (locomotion, exploration, light stress, planarian light/dark test, shoaling and foraging) conducted with three different species Dugesia tigrina, Schmidtea mediterranea and Schmidtea polychroa. The behavioral responses of every species in each of these six assays were recorded and differences between species were highlighted, depending on the assays and conditions. Schmidtea polychroa is less active than the two others and had the highest light aversion. Reactions observed in response to diverse and realistic stimuli helped us to select the most suitable tests and choose the species that seem the most appropriate for future ecotoxicological and neurophysiological tests. Four tests - out of the six tested- seem reliable in order to standardize planarian behavioral tests.

中文翻译：

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三种淡水涡虫对光、视觉和嗅觉刺激的行为反应：为进一步的生态毒理学研究奠定基础

涡虫是淡水扁虫，通常用作环境生物指标，因为它们反应灵敏且易于在实验室培养。然而，迄今为止，很少有研究描述他们的行为。这项工作旨在通过对三种不同物种 Dugesia tigrina、Schmidtea mediterranea 和 Schmidtea polychroa 进行的六项行为挑战（运动、探索、光应激、涡虫光/暗测试、浅滩和觅食）提供初步结果来填补文献空白。记录了这六个测定中每个物种的行为反应，并根据测定和条件突出了物种之间的差异。Schmidtea polychroa 比其他两个更不活跃，并且对光的厌恶程度最高。观察到的对多样化和现实刺激的反应有助于我们选择最合适的测试，并选择似乎最适合未来生态毒理学和神经生理学测试的物种。为了标准化涡虫行为测试，四项测试 - 在六项测试中 - 似乎是可靠的。