Abstract

Silk fibroin (SF) and polyhydroxyalkanoate (PHA) have received increasing attention in recent years because of their unique biodegradability and biocompatibility. Studies on SF or PHA-based materials that are intended for use as biomaterials have attracted great attention, but few studies have been conducted on SF/PHA composite materials. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) copolymers with varying ratios of the 3-hydroxyvalerate (HV) component are the most widely used member of PHAs. In this study, SF/PHBV composite nanofibers were prepared by electrospinning. The stability of the mixed solution of SF/PHBV was explored first. Fourier transform infrared (FTIR) spectroscopy was used to study the composition of the precipitates caused by mixing the SF solution with the PHBV solution. The morphology of the electrospun SF/PHBV composite nanofibers was observed by scanning electron microscopy (SEM). The average diameters of the pure PHBV, pure SF, SF/PHBV (the content ratio of solute SF/PHBV (w/w) was 10/1), and SF/PHBV (the content ratio of solute SF/PHBV (w/w) was 4/1) nanofibers were 495.12 ± 82.27, 67.88 ± 14.52, 171.10 ± 40.38, and 149.70 ± 28.57 nm, respectively. To discuss the influence of the electrospinning parameters on the spinning stability and morphology of the electrospun SF/PHBV (10/1) composite nanofibers, a univariate analysis experiment was designed. When the applied voltage was 15 kV, the feeding rate was 0.3 mL/h, and the tip-to-collector distance was 13 cm, the spinning stability, uniformity and formability of the SF/PHBV (10/1) composite nanofibers were excellent. In addition to FTIR analysis, X-ray diffraction (XRD) was also used to explore the structure of the electrospun SF/PHBV composite nanofibers. As the content of PHBV increased, the crystallinity of the SF/PHBV composite nanofibers could be improved. The thermogravimetric analysis (TGA) results indicated that the thermal stability of SF was better than that of PHBV. As the content of PHBV increased, the thermal stability of the SF/PHBV composite nanofibers would decrease.

中文翻译：

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再生丝素蛋白和PHBV电纺生物聚合物复合纳米纤维：制备方法，形态和热稳定性。

摘要

丝素蛋白（SF）和聚羟基链烷酸酯（PHA）近年来因其独特的生物降解性和生物相容性而受到越来越多的关注。旨在用作生物材料的基于SF或PHA的材料的研究引起了极大的关注，但是关于SF / PHA复合材料的研究很少。聚（3-羟基丁酸酯-co3-羟基戊酸酯（HV）组分比例不同的-3-羟基戊酸酯（PHBV）共聚物是PHA中使用最广泛的成员。在这项研究中，SF / PHBV复合纳米纤维是通过静电纺丝制备的。首先探讨了SF / PHBV混合溶液的稳定性。傅里叶变换红外（FTIR）光谱用于研究由SF溶液和PHBV溶液混合引起的沉淀物组成。通过扫描电子显微镜（SEM）观察了电纺SF / PHBV复合纳米纤维的形态。纯PHBV，纯SF，SF / PHBV（溶质SF / PHBV的含量比（w / w）为10/1）和SF / PHBV（溶质SF / PHBV的含量比（w / w） w）为4/1）纳米纤维分别为495.12±82.27、67.88±14.52、171.10±40.38和149.70±28.57 nm。为探讨电纺参数对电纺SF / PHBV（10/1）复合纳米纤维纺丝稳定性和形态的影响，设计了单变量分析实验。当施加电压为15 kV时，进料速度为0.3 mL / h，针尖到收集器的距离为13 cm，SF / PHBV（10/1）复合纳米纤维的纺丝稳定性，均匀性和可成型性非常好。除FTIR分析外，还使用X射线衍射（XRD）探索电纺SF / PHBV复合纳米纤维的结构。随着PHBV含量的增加，SF / PHBV复合纳米纤维的结晶度可以提高。热重分析（TGA）结果表明，SF的热稳定性优于PHBV。随着PHBV含量的增加，