Abstract

Nanofibers are known as promising carriers for anticancer drug delivery, particularly in postoperative local chemotherapy, through surgical grafting the membrane. In this study, blends of poly (ε‑caprolactone) (PCL) and gelatin (Ge) at various polymeric proportions were used for electrospinning of the nanofibers containing the anticancer drug of 5-Fluorouracil (5-FU). The electrospinning was performed at three different conditions to study the influence of processing parameters on the membrane properties. The morphology and tensile properties of the PCL-Ge nanofibers were reliant on the gelatin ratio and the processing condition. Fourier-transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) outcomes demonstrated that 5-Fluorouracil (5-FU) was well distributed in the fiber matrix, forming H-bonds and in an amorphous state. Weight loss measurements and drug release study showed much faster degradation, and 5-FU releasing from the nanofibers contained a higher amount of gelatin. Studying the cytotoxicity of the 5-FU-loaded nanofibers showed that the P70G30FU had well restrained the survive, attachment, and proliferation of HT-29 colorectal cancer cells. Therefore, the 5-FU loaded PCL-Ge nanofibers have the ability of controlled drug release following the desired cancer treatment protocol.

中文翻译：

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聚己内酯-明胶膜对5-氟尿嘧啶的控制药物传递

摘要

纳米纤维被认为是用于抗癌药物递送的有希望的载体，特别是在通过手术移植膜进行的术后局部化疗中。在这项研究中，聚（ε-己内酯）（PCL）和明胶（Ge）的各种聚合物比例的混合物用于静电纺丝含有5-氟尿嘧啶（5-FU）抗癌药的纳米纤维。在三种不同条件下进行静电纺丝，以研究加工参数对膜性能的影响。PCL-Ge纳米纤维的形态和拉伸性能取决于明胶比例和加工条件。傅立叶变换红外光谱（FTIR）和差示扫描量热法（DSC）的结果表明，5-氟尿嘧啶（5-FU）在纤维基质中分布良好，形成氢键并处于非晶态。重量损失测量和药物释放研究表明，降解要快得多，而从纳米纤维中释放的5-FU含有大量的明胶。研究加载5-FU的纳米纤维的细胞毒性表明，P70G30FU很好地抑制了HT-29结直肠癌细胞的存活，附着和增殖。因此，载有5-FU的PCL-Ge纳米纤维具有按照所需的癌症治疗方案控制药物释放的能力。