Abstract

How a number of biological effects of the peptide neuroprotector HLDF-6-amide work are still unknown and require further study. It was found that HLDF-6-amide dose-dependent (1–100 μM) stimulates the proliferation of Tetrahymena pyriformis infusoria, and the MK801 glutamate NMDA receptor antagonist partially blocks this effect. The introduction of a peptide (12.5–100 μM) reduces the cytotoxic and cytostatic effect of doxorubicin (25 μM) on infusoria. Radio-ligand analysis revealed the presence of specific binding sites of [3H] Ac-HLDF-6-amide on the cell membranes of the rat brain cortex, which indicates the receptor mechanism of action of the peptide. Thus, it was shown for the first time that HLDF-6-amide stimulates proliferation and has cytoprotective properties, which may underlie its wound-healing, nootropic and neuroprotective effects. The participation of the glutamate system, in particular ionotropic NMDA receptors, in these effects was established.

中文翻译：

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HLDF-6-酰胺降低阿霉素的细胞毒性并激活梨形四膜虫的增殖

摘要

肽神经保护剂HLDF-6-酰胺的许多生物学作用如何发挥仍是未知的，需要进一步研究。发现HLDF-6-酰胺剂量依赖性（1–100μM）刺激梨形四膜虫的增殖Infusoria和MK801谷氨酸NMDA受体拮抗剂可部分阻断这种作用。引入一种肽（12.5–100μM）可降低阿霉素（25μM）对输尿管的细胞毒性和抑制细胞的作用。放射性配体分析揭示了[3H] Ac-HLDF-6-酰胺在大鼠大脑皮层细胞膜上的特异性结合位点的存在，这表明该肽的受体作用机制。因此，首次显示HLDF-6-酰胺刺激增殖并具有细胞保护特性，这可能是其伤口愈合，促智和神经保护作用的基础。建立了谷氨酸系统特别是离子型NMDA受体的参与这些作用。