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The Hybrid Protein ZZ–OL as an Analytical Tool for Biotechnology Research
Russian Journal of Bioorganic Chemistry ( IF 1 ) Pub Date : 2020-12-28 , DOI: 10.1134/s106816202006014x
V. V. Krasitskaya , E. E. Bashmakova , A. N. Kudryavtsev , M. A. Vorobjeva , E. A. Shatunova , L. A. Frank

Abstract

The gene of the hybrid protein that encodes the double synthetic fragment proZZ of the immunoglobulin-binding domain of protein A of Staphylococcus aureus and apo-obelin joined by a short linker has been cloned. The corresponding hybrid protein has been obtained by expression in Esсheriсhia coli cells. The protein activated with a substrate (coelenterazine) possesses the bioluminescent Са2+-dependent activity of the photoprotein close to that of recombinant wild-type obelin, and the immunoglobulin-binding ability of protein A. It has been shown that the hybrid can be used as a highly sensitive label to detect antibodies and estimate their affinity and interaction with recombinant proteins, as well as in investigations of other kinds.



中文翻译:

杂合蛋白ZZ–OL作为生物技术研究的分析工具

摘要

已经克隆了编码金黄色葡萄球菌A蛋白和脱辅基骨蛋白通过短接头连接的免疫球蛋白结合结构域的双合成片段proZZ的杂合蛋白的基因。通过在大肠杆菌细胞中表达获得了相应的杂合蛋白。被底物激活的蛋白质(腔肠素)具有与重组野生型鄂贝林接近的光蛋白的生物发光Са2 +依赖性活性,以及​​蛋白A的免疫球蛋白结合能力。用作高度敏感的标记,可检测抗体并评估其与重组蛋白的亲和力和相互作用,以及用于其他种类的研究。

更新日期:2020-12-28
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