Abstract

The aim of the present study has been to evaluate the antioxidant, antimicrobial and wound healing activity of Algerian Lavandula stoechas and Mentha pulegium methanol extracts (LE and ME, respectively) in order to scientifically validate their traditional use. After a preliminary phytochemical screening, a polyphenol profile characterization was carried out by RP-LC-DAD-ESI-MS/MS analysis. Antioxidant activity was evaluated by different in vitro assays such as DPPH, TEAC, FRAP and ORAC whereas antimicrobial activity was carried out against representative standard ATCC and clinical Gram-positive and Gram-negative strains as well as against Candida albicans. The wound healing activity of ointments containing 5% and 10% of LE and ME was investigated on Wistar albino rats. Different polyphenol classess (flavones, flavonols and phenolic acids) were identified with rosmarinic acid that was the most abundant compound in both extracts (5658.40 and 1201.35 mg/100 g of LE and ME, respectively). LE and ME have strong antioxidant activity, whereas they showed antimicrobial activity only against S. aureus. However, both 5% and 10% LE and ME ointments significantly accelerated the wound healing process in comparison with negative control, providing a scientific rationale for the traditional use of L. stoechas and M. pulegium aerial parts in the treatment of wounds.

摘要

本研究的目的是评估阿尔及利亚Lavandula stoechas和Mentha pulegium甲醇提取物（分别为 LE 和 ME）的抗氧化、抗菌和伤口愈合活性，以科学地验证它们的传统用途。在初步植物化学筛选后，通过 RP-LC-DAD-ESI-MS/MS 分析进行多酚谱表征。抗氧化活性通过不同的体外试验评估，如 DPPH、TEAC、FRAP 和 ORAC，而抗微生物活性是针对代表性标准 ATCC 和临床革兰氏阳性和革兰氏阴性菌株以及白色念珠菌进行的. 在 Wistar 白化大鼠身上研究了含有 5% 和 10% LE 和 ME 的软膏的伤口愈合活性。用迷迭香酸鉴定出不同的多酚类别（黄酮、黄酮醇和酚酸），迷迭香酸是两种提取物中最丰富的化合物（LE 和 ME 分别为 5658.40 和 1201.35 mg/100 g）。LE 和 ME 具有很强的抗氧化活性，而它们仅对金黄色葡萄球菌具有抗菌活性。然而，与阴性对照相比，5% 和 10% LE 和 ME 软膏均显着加速了伤口愈合过程，为传统使用L. stoechas和M. pulegium地上部分治疗伤口提供了科学依据。