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Dynamic changes in monocytes subsets in COVID-19 patients
Human Immunology ( IF 2.7 ) Pub Date : 2020-12-26 , DOI: 10.1016/j.humimm.2020.12.010
Shuang Qin , Yujie Jiang , Xin Wei , Xiaoyuan Liu , Jingjing Guan , Yingxiao Chen , Hong Lu , Jingjing Qian , Zhongyong Wang , Xiangyang Lin

Background

Coronavirus disease 2019 (COVID-19) is affecting the whole world and threatening human health. We aim to investigate the immunological characteristics of monocytes in critical patients with COVID-19.

Methods

The number and immune status of monocytes were detected by flow cytometry in 32 COVID-19 patients and 18 healthy individuals.

Results

In critical patients with COVID-19, the absolute number of total monocytes and CD16 monocytes was significantly decreased but CD16+ pro-inflammatory monocytes was increased compared to healthy controls. Antigen presentation potential of monocytes, as measured by HLA-DR expression, was suppressed, while their inflammatory phenotype (CD38 expression) was enhanced. Cytokine levels showed sustained increases in critical patients. And the levels of IL-6 were positively correlated with CD16+ monocytes number. IL-6 and IL-10 levels were negatively correlated with HLA-DR expression of monocytes. During the recovery of COVID-19 patients, the count and immune status of monocyte subsets were restored by degrees. HLA-DR+ monocytes possessed good sensitivity and specificity for predicting the incidence of critical patients with COVID-19.

Conclusions

In critical patients with COVID-19, decline in number and HLA-DR expression of monocytes might lead to decreased antigen presentation potential and thus immunosuppression, while increased CD16+ pro-inflammatory monocytes might mediate hyperinflammation. HLA-DR+ monocytes might be a meaningful assisted indicator to predict the incidence of critical patients with COVID-19.



中文翻译:

COVID-19患者单核细胞亚群的动态变化

背景

2019年冠状病毒病(COVID-19)正在影响整个世界并威胁着人类健康。我们旨在研究重症患者COVID-19的单核细胞免疫学特征。

方法

通过流式细胞术检测了32例COVID-19患者和18例健康个体的单核细胞数量和免疫状态。

结果

危重病患者COVID-19,总单核细胞和CD16的绝对数量-单核细胞显著下降,但CD16 +健康对照组相比促炎性单核细胞增加。通过HLA-DR表达测量,单核细胞的抗原呈递潜力受到抑制,而其炎症表型(CD38表达)则得到增强。细胞因子水平显示危重患者持续增加。IL-6的水平与CD16 +单核细胞数量呈正相关。IL-6和IL-10水平与单核细胞的HLA-DR表达负相关。在COVID-19患者的恢复过程中,单核细胞亚群的计数和免疫状态按程度恢复。HLA-DR + 单核细胞具有良好的敏感性和特异性,可预测重症COVID-19患者的发病率。

结论

在严重的COVID-19患者中,单核细胞数量减少和HLA-DR表达下降可能会导致抗原呈递潜能降低,从而导致免疫抑制,而CD16 +促炎性单核细胞增多可能会介导过度炎症。HLA-DR +单核细胞可能是预测COVID-19危重患者发病率的有意义的辅助指标。

更新日期:2021-02-23
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