Tree-ring stable oxygen and carbon isotope ratios (δ¹⁸O and δ¹³C) are an important archive for climate reconstructions. However, it remains unclear whether the polyvinyl acetate emulsion, often used for the preservation and fixation of wood samples, influences δ¹⁸O and δ¹³C signals. Further uncertainties are associated with the possible effects of geographical origin and cambial age of historical samples. Here, we present annually-resolved and absolutely-dated δ¹⁸O and δ¹³C measurements of 21 living oaks (Quercus robur and Q. petraea) from the Czech Republic. We find that the δ¹⁸O and δ¹³C signals in the extracted alpha-cellulose are not affected by polyvinyl acetate treatment. Covering the entire 20^th century and reaching until 2018 CE, our dataset reveals spatial and temporal coherency within and between the individual δ¹⁸O and δ¹³C chronologies of different oak species, sample locations, and tree ages. Highly significant (p < 0.01) Pearson’s correlation coefficients of the site-specific δ¹³C and δ¹⁸O chronologies range from 0.48–0.77 and 0.36–0.56, respectively. The isotopic inter-series correlations of Q. robur and Q. petraea from the same site are 0.75 and 0.43 for the mean δ¹³C and δ¹⁸O values, respectively. Significant (p < 0.01) correlations of 0.49 and 0.84 are found for δ¹³C and δ¹⁸O, respectively, when all measurements from all sampling locations and tree ages are included. Our study shows that non-pooled oak δ¹⁸O and δ¹³C measurements from both species, different locations, and diverse tree ages can be combined into robust isotopic chronologies for climate reconstructions.

年轮稳定氧和碳同位素比率（δ ¹⁸ O和δ ¹³ C）是一个重要的气候重建归档文件。然而，聚乙酸乙烯酯乳液，通常用于保存和木材样品的固定是否影响δ仍不清楚¹⁸ O和δ ^13个C信号。进一步的不确定性与地理来源和历史样本的冈比亚年龄的可能影响有关。这里，我们目前每年分辨和绝对过时δ ¹⁸ O和δ ^13个21个活橡树（C测量夏栎和Q.佩特拉行省来自捷克共和国）。我们发现δ ¹⁸ O和δ ¹³提取的α-纤维素中的C信号不受聚乙酸乙烯酯处理的影响。覆盖了整个20^个世纪，直到达到2018 CE，我们的数据显示内与个体之间δ时空一致性¹⁸ O和δ ^13个不同橡木品种，采样地点和树龄的Ç年表。高度显著（p <0.01）的位点特异性的的δPearson相关系数¹³ C和δ ^18个ö年表范围从分别0.48-0.77和0.36-0.56。的同位素间系列的相关性Q.栎和Q.佩特拉行省来自同一网站是0.75和0.43的平均δ ¹³ C和δ分别为¹⁸ O值。显著（p的0.49和0.84 <0.01）的相关性被发现对于δ ¹³ C和δ ¹⁸ O，分别当包括从所有采样位置和树龄所有测量。我们的研究表明，非池橡木δ ¹⁸ O和δ ^13个来自两个物种，不同地点和不同树龄ç测量可以组合成气候重建稳健的同位素年代学。