Abstract The choosing of micromechanical environment is very important for the growth of bone-related cells. In this paper, bovine cancellous bone slices with 3D porous structures were used for 3D culturing of MC3T3-E1 cells (Mouse embryo osteoblast precursor cells) through a four-point-bending device due to their good biocompatibility and strength. Effects of micromechanical environment on the growth of MC3TC-E1 cells were investigated by immunofluorescent staining and alkaline phosphatase analysis, and the most positive microporous structures were found. In addition, a model of cell density vs stress was established through a specific normalization method and finite element simulation. The results showed that the micromechanical environment of the bone slices promoted cell proliferation, and the detail influence of stress on cell proliferation could be described by the mathematical model, which could provide a theoretical basis for the design of micromechanical environment in the bone tissue engineering scaffolds to stimulate cell proliferation.

中文翻译：

---

应激对 MC3T3-E1 细胞在微孔牛骨切片上的 3D 培养的影响

摘要 微机械环境的选择对骨相关细胞的生长非常重要。在本文中，具有 3D 多孔结构的牛松质骨切片由于其良好的生物相容性和强度，通过四点弯曲装置用于 MC3T3-E1 细胞（小鼠胚胎成骨细胞前体细胞）的 3D 培养。通过免疫荧光染色和碱性磷酸酶分析研究了微机械环境对MC3TC-E1细胞生长的影响，发现了最阳性的微孔结构。此外，通过特定的归一化方法和有限元模拟建立了细胞密度与应力的模型。结果表明，骨切片的微机械环境促进了细胞增殖，