Little is known about the molecular responses to power resistance exercise that lead to skeletal muscle remodeling and enhanced athletic performance. We assessed the expression of titin-linked putative mechanosensing proteins implicated in muscle remodeling: muscle ankyrin repeat proteins (Ankrd1, Ankrd2 and Ankrd23), muscle-LIM proteins (MLP), muscle RING-finger protein-1 (MuRF-1), and associated myogenic proteins (MyoD1, myogenin, and myostatin) in skeletal muscle in response to power resistance exercise with or without a post-exercise meal, in fed, resistance-trained men. A muscle sample was obtained from the vastus lateralis of seven healthy men on separate days, 3h after 90 min of rest (Rest) or power resistance exercise with (Ex+Meal) or without (Ex) a post-exercise meal, to quantify mRNA and protein levels. The levels of phosphorylated HSP27 (pHSP27-Ser15) and cytoskeletal proteins in muscle and creatine kinase activity in serum were also assessed. The exercise increased (P≤0.05) pHSP27-Ser15 (~6-fold) and creatine kinase (~50%), whereas cytoskeletal protein levels were unchanged (P>0.05). Ankrd1 (~15-fold) and MLP (~2-fold) mRNA increased, whereas Ankrd2, Ankrd23, MuRF-1, MyoD1, and myostatin mRNA were unchanged. Ankrd1 (~3-fold, Ex) and MLPb (~20-fold, Ex+Meal) protein increased, but MLPa, Ankrd2, Ankrd23, and the myogenic proteins were unchanged. The post-exercise meal did not affect the responses observed. Power resistance exercise, as performed in practice, induced subtle early responses in the expression of MLP and Ankrd1, yet had little effect on the other proteins investigated. These findings suggest possible roles for MLP and Ankrd1 in the remodeling of skeletal muscle in individuals who regularly perform this type of exercise.

中文翻译：

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力抗运动的男性中，力竭运动后骨骼肌中与肌动蛋白连锁的假定的机械传感蛋白的表达

关于抵抗力运动导致骨骼肌重塑和增强运动表现的分子反应知之甚少。我们评估了与肌肉重塑有关的与蛋白连接的推定性机械传感蛋白的表达：肌肉锚蛋白重复蛋白（Ankrd1，Ankrd2和Ankrd23），肌肉LIM蛋白（MLP），肌肉RING-手指蛋白1（MuRF-1）和饮食中受过抗性训练的男性中，有或无运动后进餐的力量抵抗运动对骨骼肌中相关的肌源性蛋白质（MyoD1，肌生成素和肌生成抑制素）的影响。分别在休息（休息）或力量抵抗运动（运动后进餐（Ex + Meal）或不运动（Ex））90分钟后3小时，从7位健康男性的股外侧肌获得肌肉样本以定量mRNA和蛋白质水平。还评估了血清中肌肉和肌酸激酶活性中磷酸化的HSP27（pHSP27-Ser15）和细胞骨架蛋白的水平。运动增加（SP≤0.05）pHSP27-Ser15（〜6倍）和肌酸激酶（〜50％），而细胞骨架蛋白水平不变（P> 0.05）。Ankrd1（〜15倍）和MLP（〜2倍）mRNA增加，而Ankrd2，Ankrd23，MuRF-1，MyoD1和myostatin mRNA不变。Ankrd1（〜3倍，Ex）和MLPb（〜20倍，Ex + Meal）蛋白增加，但MLPa，Ankrd2，Ankrd23和成肌蛋白未改变。运动后进餐不影响观察到的反应。在实践中进行的力量抵抗运动在MLP和Ankrd1的表达中引起了微妙的早期反应，但对其他研究的蛋白质几乎没有影响。